Sensory, Nutritional, Functional, Flavour Compounds and Microbial Properties of Novel Seasoning Cubes Produced from Tree Tomato Fruit and Crayfish as Influenced byFermentation and Drying

Authors

  • Maboh J Centre for Food Technology and Research, Rev Fr. Moses Orshio Adasu University, Makurdi, Benue State, Nigeria
  • Eke M.O Department of Food Science and Technology, Joseph Sarwuan Tarka University, Makurdi, Benue State, Nigeria
  • Okibe F. G. Department of Chemistry, Federal University of Health Sciences, Otukpo, Benue State, Nigeria

DOI:

https://doi.org/10.54536/ajfst.v5i2.7434

Keywords:

Crayfish, Fermentation Drying, Flavour Compounds, Functional, Microbial Properties, Nutritional, Seasoning Cubes, Sensory Analysis, Tree Tomato

Abstract

This study evaluated the effect of fermentation and drying on the core quality attributes of seasoning cubes produced from tree tomato and crayfish blends. Preliminary sensory evaluation was performed on five samples of salted and non-salted seasoning cubes containing varying percentages of tree tomato and crayfish. Preliminary results showed that the salted sample containing 60% tree tomato and 40% crayfish, and the non-salted sample containing 70% tree tomato and 30% crayfish, were most preferred. Portions of these two samples were further subjected to fermentation, drying and fermentation-drying. Sensory results showed that fermented-dried salted seasoning (FDSS) and fermented non-salted (FNSS) samples were the most preferred, with 8.30 and 6.80 acceptabilities, respectively. Proximate analysis revealed FDSS had the highest protein (26.38%) and energy content (394.23 kcal), significantly surpassing the commercial control sample (CCS) (6.98% protein, 354.64 kcal). Functional properties showed FDSS had high oil absorption (2.46 g/g) and bulk density (0.52 g/mL), while FNSS exhibited superior dispersibility (58.35%). GC-MS analysis detected up to thirty-two volatile flavour compounds in FDNSS (fermented and dried non-salted seasoning), including terpenes, esters, aldehydes, and phenolics, compared to only two in CCS. Microbial analysis indicated acceptable bacterial loads (<10⁴ CFU/g) across all samples but elevated fungal counts (up to 7.92 × 10³ CFU/g in FNSS), exceeding the recommended limit (<10³ CFU/g). Salt significantly suppressed microbial growth and enhanced sensory attributes, while fermentation enriched flavour complexity and nutritional value. From the results, it could be concluded that the synergistic application of fermentation and drying significantly improved the nutritional profile, flavour diversity, and functional performance of tree tomato–crayfish seasoning cubes. FDSS emerged as the optimal formulation for the salted samples and FNSS for the non-salted samples, balancing high protein, palatability, and safety, supporting the development of clean-label, nutrient-dense alternatives to synthetic commercial seasonings, aligning with consumer demand for more natural and healthy products.

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References

Adebo, O. A., & Medina-Meza, I. (2023). Impact of fermentation on the physicochemical and moisture sorption properties of plant-based protein seasonings. Food Chemistry, 405, Part A, 134821. https://doi.org/10.1016/j.foodchem.2022.134821

Akhigbemidu, W., Musa, A. & Kuforiji, O. (2015). Assessment of the microbial qualities of noodles and the accompanying seasonings. Nigerian Food Journal, 33, 48–53. https://doi.org/10.1016/j.nifoj.2015.04.005

Anukam, K. C., Osazuwa, F., & Ahmed, K. (2021). Microbiological and safety evaluation of fermented ogiri-seed condiments in Nigeria. African Journal of Biotechnology, 20(4), 145–152. https://doi.org/10.5897/AJB2020.17123

AOAC. (2015). Offcial methods of Analysis (18th ed.).

Asioli, D., Aschemann-Witzel, J., Caputo, V., Vecchio, R., Annunziata, A., Næs, T. & Varela, P. (2017). Making sense of the “clean label” trends: A review of consumer food choice behavior and discussion of industry implications. Food Research International, 99, 58–71. https://doi.org/10.1016/j.foodres.2017.07.022

Awuchi, C. G. (2019). Proximate Composition and Functional Properties of Different Grain Flour Composites for Industrial Applications. International Journal of Food Sciences, 2(1), 43–64.

Brian, N. P. (2022). Characterization and Utilization of Flavors from Undersized Crawfish. https://doi.org/10.31390/gradschool_disstheses.6620

Cao, Y. & Miao, L. (2023). Consumer perception of clean food labels. British Food Journal, 125(2), 433–448.

Chen, X., Li, Y., Wang, J., & Zhang, H. (2021). Effect of fermentation on the functional properties and antioxidant activity of soy sauce. Food Chemistry, 340, 128089. https://doi.org/10.1016/j.foodchem.2020.128089

Davila, M. (2023). Exploring the world of flavour enhancers: understanding their uses and benefits in food. American Journal of Food Science and Technology, 008.

de Mejia, E. G., Aguilera‐Gutiérrez, Y., Martin‐Cabrejas, M. A., & Mejia, L. A. (2015). Industrial processing of condiments and seasonings and its implications for micronutrient fortification. Annals of the New York Academy of Sciences, 1357(1), 8-28.

Emurotu, J. E., & Adegbe, A. A. (2017). Physicochemical properties and some heavy metal content in some spices and seasonings sold in a market near Obajana Cement Company, Kogi State, Nigeria. International Research Journal of Environmental Sciences, 6(7), 9–12.

Fadimatou, B., Bonheur, B. & Loh, A. M. B. (2024). Sensory and Nutritional Properties and Stability of Formulated Organic Food Flavour Enhancers. Open Journal of Applied Sciences, 14(6), 1490-1506.

García, M., Martínez, A., & Pérez, J. (2023). Impact of salting on the solubility and sensory properties of seasoned meat products. Journal of Food Science and Technology, 60(1), 123–132. https://doi.org/10.1007/s13197-022-05567-8

Ge, Y., Wu, Y., Aihaiti, A., Wang, L., Wang, Y., Xing, J., Zhu, M., & Hong, J. (2025). The Metabolic Pathways of Yeast and Acetic Acid Bacteria During Fruit Vinegar Fermentation and Their Influence on Flavor Development. Microorganisms, 13(3), 477. https://doi.org/10.3390/microorganisms13030477

Hong, P., Ndagijimana, M., & Betti, M. (2016). Glucosamine-induced glycation of hydrolysed meat proteins in the presence or absence of transglutaminase: Chemical modifications and taste-enhancing activity. Food chemistry, 197, 1143-52. https://doi.org/10.1016/j.foodchem.2015.11.096.

International Commission on Microbiological Specifications for Foods. (2022). Microorganisms in foods 7: Microbiological testing in food safety management (2nd ed.). Springer.

Iwe, A. N., Onyeukwu, M. O., & Agiriga, U. (2016). Proximate, functional and pasting properties of FARO 44 rice, African yam bean, and brown cowpea seeds composite flour. Cogent Food & Agriculture, 2(1), Article 1142409. https://doi.org/10.1080/23311932.2016.1142409

Jayaraman, K. S., & Gupta, D. D. (2020). Drying of fruits and vegetables. In Handbook of industrial drying (pp. 643-690). CRC Press.

Johnson, L., & Lee, K. (2021). Evaluating the impact of salt on seafood-based seasoning cubes. Food Chemistry, 345, 128765. https://doi.org/10.1016/j.foodchem.2021.128765

Kane, A., Sow, A., Ndiaye, N. A., Sagna, C.G., Diakhaté, P. A., Cissé, M. & Diop, A. (2024) Sweetener and Flavor Enhancer Food Additives in Industrial Food Products Marketed in Dakar: Frequency and Diversity. Open Journal of Applied Sciences, 14, 101-117. https://doi.org/10.4236/ojapps.2024.141008

Kaur, M., & Sandhu, K. S. (2016). Sweet potato flour and starch. In V. R. Preedy (Ed.), Tropical and subtropical roots: Chemistry, technology, and human nutrition (pp. 479–506). Wiley. https://doi.org/10.1002/9781118992739.ch11a

Kefale, B., Delele, M. A., Fanta, S. W., & Abate, S. M. (2023). Nutritional, physicochemical, functional, and textural properties of red pepper (Capsicum annuum L.), red onion (Allium cepa), ginger (Zingiber officinale), and garlic (Allium sativum): Main ingredients for the preparation of spicy foods in Ethiopia. Journal of Food Quality, 2023, Article 3916692. https://doi.org/10.1155/2023/3916692

Kong, J. M., Chia, L. S., Goh, N. K., Chia, T. F., & Brouillard, R. (2003). Analysis and biological activities of anthocyanins. Phytochemistry, 64(5), 923–933. https://doi.org/10.1016/S0031-9422(03)00438-2

Kumar, S., & Singh, R. P. (2020). Drying techniques and their impact on the functional properties of fermented foods. Critical Reviews in Food Science and Nutrition, 60(14), 2455–2472. https://doi.org/10.1080/10408398.2019.1698321

Li, J., Zhang, Y., & Wang, Q. (2022). Role of salt in enhancing the texture and functional properties of plant-based meat analogues. Food Hydrocolloids, 125, 107189. https://doi.org/10.1016/j.foodhyd.2022.107189

Li, X., Han, T., Zheng, S. & Wu, G. (2021). Nutrition and Functions of Amino Acids in Aquatic Crustaceans. In G. Wu (Ed.), Amino Acids in Nutrition and Health: Amino Acids in the Nutrition of Companion, Zoo and Farm Animals (pp. 169–198). Springer International Publishing. https://doi.org/10.1007/978-3-030-54462-1_9

Ma, F., Li, Y., Zhang, Y., Zhang, Q., Li, X., Cao, Q. & Liu, G. (2024). Effects of umami substances as taste enhancers on salt reduction in meat products: A review. Food Research International, 114248.

Maruyama, S., Streletskaya, N. A. & Lim, J. (2021). Clean label: Why this ingredient but not that one? Food Quality and Preference, 87, 104062.

Massamba, S. M., Elenga, M., Lébonguy, A. A., & Zébita, G. R. M. (2024). Organoleptic and microbiological characteristics of cubes of Irvingia gabonensis almonds (Péké). African Journal of Food Science, 18(3), 48–59. https://doi.org/10.5897/AJFS2024.2299

Mohd, A. U. N., Khairil, M. N. F., Raja N., R. M. H., Yuswan, M. H., Mohd, H. A., Abbasiliasi, S., Mohd, D. M. N., Ismail, A., & Mustafa, S. (2021). Mass spectrometry determination of potential species-specific peptide markers in commercial seasoning cubes. Journal of Food Composition and Analysis, 104, Article 104193. https://doi.org/10.1016/j.jfca.2021.104193

Moretti, D., Hurrell, R. F., & Cercamondi, C. I. (2018). Bouillon cubes. In M. G. V. Mannar & R. F. Hurrell (Eds.), Food fortification in a globalized world (pp. 159–165). Academic Press. https://doi.org/10.1016/B978-0-12-802861-2.00016-X

Morton, J. F. (2013). Fruits of warm climates. Creative Resource Systems.

Ndife, J., Linus-Chibuezeh, A., Adindu-Linus, C. O., & Ugwunebo, A. C. (2022). Spices: Uziza (Piper guineense), Uda (Xylopia aethiopica), Ehuru (Monodora myristica), and ginger (Zingiber officinale). FUTO Journal Series, 6(2), 174–189.

Niaz, K., Zaplatic, E., & Spoor, J. (2018). Extensive use of monosodium glutamate: A threat to public health? EXCLI Journal, 17, 273–278. https://doi.org/10.17179/excli2018-1092

Njoya, M. A., Nain, C. W., Nde, S. C., Mahbou, P. Y. & Imele, H. (2020). Physicochemical and sensory properties of tree tomato (Cyphomandra betacea (Cav.) Sendtner) drink. World Journal of Advanced Research and Reviews, 6(2), 009–016. https://doi.org/10.30574/wjarr.2020.6.2.0068

Nkeudem, A. G., Irene, S., Ngole, U., Judith, A., Ning, T. R., Metuge, S. & Giscard, K. K. (2018). Nutritional Evaluation of Commonly Used Local Weaning Food Processed and Sold in the Mount Cameroon Region, 8(6), 131–141. https://doi.org/10.5923/j.food.20180806.01

Nwachukwu, I. E., Ezeama, C. F., & Okafor, N. (2023). Development and quality evaluation of composite seasoning cubes from fermented crayfish and tomato. Journal of Food Science and Technology, 60(2), 789–798. https://doi.org/10.1007/s13197 022-05578-

Nwankwo, R. N., & Omar, A. W. H. (2021). Comparative analysis of ingredients, nutrient composition and consumption pattern of commonly consumed bouillon cubes in Mubi North Local Government Area, Adamawa State, Nigeria. African Journal of Agricultural Science and Food Research, 13(1).

Ojinnaka, M. C., & Agubolum, F. U. (2013). Nutritional and sensory properties of cashew nut–wheat based cookies. American Journal of Food and Nutrition, 3(3), 127–134.

Oksuz, A. & Mazlum, Y. (2016). Determination of proximate composition and fatty acid profiles of Astacus leptodactylus Eschscholtz, 1823 in Turkish freshwater resources. Crustaceana, 89(10), 1135–1147.

Oladiran, O., Adebowale, K., & Adewuyi, O. (2021). Effect of drying methods on the moisture content and shelf life of food products. Drying Technology, 39(10), 1234–1245. https://doi.org/10.1080/07373937.2021.1892345

Olaide, R. A., Chizobam, H. I., Sunday, F. A., & Sturm, B. (2020, September 9–11). Nutrient and antinutrient composition of bouillon cubes developed from fermented condiments of Ricinus communis L. seeds. In Conference on International Research on Food Security, Natural Resource Management and Rural Development, ATSAF e.V., Germany.

Olasupo, N. A., Okunlola, O., & Oguntoyinbo, F. A. (2019). Salt as a modulator of functional microbiota in fermented foods: A review. International Journal of Food Microbiology, 306, 108258. https://doi.org/10.1016/j.ijfoodmicro.2019.108258

Olasupo, N. A., & Okorie, P. C. (2019). African fermented food condiments: Microbiology impacts on their nutritional values. In Frontiers and new trends in the science of fermented food and beverages. IntechOpen.

Osorio, C., Hurtado, N., Dawid, C., Hofmann, T., Heredia-Mira, F. J., & Morales, A. L. (2012). Chemical characterisation of anthocyanins in tamarillo (Solanum betaceum Cav.) and Andes berry (Rubus glaucus Benth.) fruits. Food Chemistry, 132, 1915–1921.

Oyedeji, A., Odeyemi, B., Adewuyi, S., & Azeez, L. (2021). Physical properties, chemical composition and nutritional quality potentials of Lonchocarpus sericeus (cube root) seeds and seed oil. Walailak Journal of Science and Technology, 18, 1–20.

Peng, G., Sun, J., Peng, B., Tan, Y., Wu, Y. & Bai, X. (2021). Assessment of essential element accumulation in red swamp crayfish (Procambarus clarkii) and the highly efficient selenium enrichment in freshwater animals. Journal of Food Composition and Analysis, 101, Article 103953.

Pico, J., Khomenko, I., Capozzi, V., Navarini, L., Bernal, J., Gómez, M., & Biasioli, F. (2018). Analysis of volatile organic compounds in crumb and crust of different baked and toasted gluten-free breads by direct PTR-ToF-MS and fast-GC-PTR-ToF-MS. Journal of Mass Spectrometry, 53(9), 893–902. https://doi.org/10.1002/jms.4258

Rodrigues, É. de F., Pantoja, L. de A., Soares, M. B., Nelson, D. L., & Soares dos Santos, A. (2016). Development of bouillon cubes from souari nut pulp: Formulation and physicochemical and sensorial evaluations. Brazilian Journal of Food Technology, 19. http://dx.doi.org/10.1590/1981-6723.5415

Sá, A. G. A., Carmo, L. M., & Valencia, G. A. (2023). The influence of clean food labels on consumers’ perception. Packaging Technology and Science, 36(9), 757–766. https://doi.org/10.1002/pts.2757

Saguy, I. S., Roos, Y. H., & Cohen, E. (2018). Food engineering and food science and technology: Forward-looking journey to future new horizons. Innovative Food Science & Emerging Technologies, 47, 326–334.

Sarkar, N. S., & Choudhury, S. (2017). Algae as a source of natural flavor enhancers: A mini review. Plant Science Today, 4(4), 172–176.

Sengev, I. A., Nwobi, I., & Sule, S. (2016). Effect of crayfish inclusion on the chemical and sensory properties of ogi prepared from maize, millet and sorghum. International Journal of Nutrition and Food Sciences, 5(6), 378–383. https://doi.org/10.11648/j.ijnfs.20160506.12

Shah, R. A., Bakshi, P., Itoo, H., & Kour, G. (2022). Tamarillo (Cyphomandra betacea (Cav.)) origin, cultivation, breeding and management. In Tropical plant species and technological interventions for improvement. IntechOpen.

Singh, R., Patel, M., & Mehta, S. (2022). Fiber content in food seasonings: A review. Critical Reviews in Food Science and Nutrition, 62(10), 2123–2138. https://doi.org/10.1080/10408398.2021.1901234

Śmiechowska, M., Newerli-Guz, J., & Skotnicka, M. (2021). Spices and seasoning mixes in European Union: Innovations and ensuring safety. Foods, 10(10), 2289. https://doi.org/10.3390/foods10102289

Smith, J., & Doe, A. (2020). Enhancing the sensory profile of citrus-based seasoning cubes through the use of salt. Journal of Food Science, 85(4), 1234–1245. https://doi.org/10.1111/1750- 3841.14567

Tușa, F. D., Moldovan, Z., Schmutzer, G., Magdaş, D. A., & Dehelean, A. (2012). Analysis of flavor compounds by GC/MS after liquid–liquid extraction from fruit juices. AIP Conference Proceedings, 1425, 53. https://doi.org/10.1063/1.3681965

Valente, A., Albuquerque, T. G., Sanches-Silva, A., & Costa, H. S. (2011). Ascorbic acid content in exotic fruits: A contribution to produce quality data for food composition database. Food Research International, 44, 2237–2242.

Vasilakī, A., Panagiotopoulou, E., Koupantsis, T., Katsanidis, E., & Mourtzinos, I. (2022). Recent insights in flavor enhancers: Definition, mechanism of action, taste-enhancing ingredients, analytical techniques and the potential of utilization. Critical Reviews in Food Science and Nutrition. https://doi.org/10.1080/10408398.2021.1939264

Wijayasekara, K., & Wansapala, J. (2021). Comparison of a flavour enhancer made with locally available ingredients against commercially available monosodium glutamate. International Journal of Gastronomy and Food Science, 23, 100286. https://doi.org/10.1016/j.ijgfs.2020.100286

World Health Organization. (2017). Guideline: Daily iron supplementation in infants and children. WHO Press.

Xing, Z., Fu, X., Huang, H., Xu, Y., Wei, L., Shan, C., & Du, Y. (2025). Recent advances in Lactobacillus plantarum fermentation in modifying fruit-based products: Flavor property, bioactivity, and practical production applications. Comprehensive Reviews in Food Science and Food Safety, 24(2), e70160. https://doi.org/10.1111/1541-4337.70160

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Published

2026-07-20

How to Cite

Maboh , J., Eke , M., & Okibe , F. G. (2026). Sensory, Nutritional, Functional, Flavour Compounds and Microbial Properties of Novel Seasoning Cubes Produced from Tree Tomato Fruit and Crayfish as Influenced byFermentation and Drying. American Journal of Food Science and Technology, 5(2), 30-48. https://doi.org/10.54536/ajfst.v5i2.7434

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