Role of HIV Infection in Multi-Drug Resistant Tuberculosis in Parts of Benue State, Nigeria

Authors

  • Lan Abraham Terna Department of Microbiology, Federal University of Agriculture, Makurdi, Nigeria
  • Amuta U Elizabeth Department of Zoology, Federal University of Agriculture, Makurdi, Nigeria
  • Terzungwe T Sar Department of Microbiology, Federal University of Agriculture, Makurdi, Nigeria

Keywords:

Bacteriophage, Isoniazid, Multi-Drug Resistance, Mycobacterium Tuberculosis, Rifampicin

Abstract

The pathogenesis of Tuberculosis shows that M. tuberculosis target and persist within phagocytes including T-lymphocytes in blood circulation. As a result, the possibility of cellular interaction between M. tuberculosis and HIV, especially for patients that are co-infected with HIV and TB, and subsequent exchange of genetic material via transduction needs to be investigated. Three hundred and eighty sputum samples mostly from suspected rifampicin-resistance patients were collected from Nigerian Airforce (NAF) Hospital Makurdi, and Federal Medical Centre (FMC) Makurdi. In vitro culture of sputum samples, Drugs Susceptibility Testing (DST) of M. tuberculosis isolates, and transduction protocol were carried out at the National Tuberculosis and Leprosy Training Centre (NTBLTC) Zaria, Nigeria. Statistical analysis was carried out using Student’s t-test. Minitab version 14.0 statistical software was used for data analysis. P-values < 0.05 were considered significant. Twenty-six (9.7%) cases of Multi-drug resistant tuberculosis (MDR-TB) were detected (retreated cases 7.1%; treatment naive 2.6%). Twenty-one (80.8%) were males and 5(19.2%) were females. There was statistical difference in MDR-TB between male and female in Benue State (P<0.05). The mean age group 35-45 years had the highest cases of MDR-TB accounting for 35% of MDR-TB. Human-immunodeficiency virus and Tuberculosis co-infected patients (category I) had the highest MDR-TB incidence of 10(38.5%). There was no significant difference between category-1 and category-III [patient with only TB disease (P>0.05)]. However, there were elevated cases of MDR-TB in category-III patients from 7(26.9%) to 10(38.5%) following transduction protocol. Multi-drugs resistant tuberculosis is prevalent in Benue State, affecting the most economically active youths within the age group of 35-45 years, as a result the need to direct more attention on molecular basis for M. tuberculosis drugs resistance is therefore advocated, in order to win the war against multi-drug resistant tuberculosis.

Downloads

Download data is not yet available.

References

Akaninyene, O., Victor, U., Abdulrazak, H., Soter, A., & Lawson, L. (2013). Clinical Study of Drug Resistance among Pulmonary Tuberculosis Patients in Calabar, Nigeria. Pulmonary Medicine, 10,10-16.

American Thoracic Society and Centers for Disease Control and Prevention. (2000). Diagnostic standards and classification of tuberculosis in adults and children. American Journal Respiratory Critical Care Medecine, 161(4), 1376–1395.

Bardarov, S., Bardarov Jr, S., Pavelka Jr, M. S., Sambandamurthy, V., Larsen, M., Tufariello, J., ... & Jacobs Jr, W. R. (2002). Specialized transduction: an efficient method for generating marked and unmarked targeted gene disruptions in Mycobacterium tuberculosis, M. bovis BCG and M. smegmatis. Microbiology, 148(10), 3007-3017.

Cegielski, J. P. (2010). Extensively drug-resistant tuberculosis:“there must be some kind of way out of here”. Clinical Infectious Diseases, 50(3), S195-S200.Cepheid GeneXpert MTB-RIF Assay G4 Version 5 operation manual, 2014.

David, H. L. (1970). Probability distribution of drug-resistant mutants in unselected populations of Mycobacterium tuberculosis. Applied microbiology, 20(5), 810-814.

Dean, A. S., Zignol, M., Falzon, D., Getahun, H., & Floyd, K. (2014). HIV and multidrug-resistant tuberculosis: overlapping epidemics. European Respiratory Journal, 44(1), 251-254.

Forbes, B. A., Sahm, D. F., & Weissfeld, A. S. (2007). Diagnostic microbiology (pp. 288-302). St Louis: Mosby.

Ford, C. B., Lin, P. L., Chase, M. R., Shah, R. R., Iartchouk, O., Galagan, J., ... & Fortune, S. M. (2011). Use of whole genome sequencing to estimate the mutation rate of Mycobacterium tuberculosis during latent infection. Nature genetics, 43(5), 482-486.

Idigbe, E. O., Duque, J. P., John, E. K., & Annam, O. (1992). Resistance to antituberculosis drugs in treated patients in Lagos, Nigeria. The Journal of Tropical Medicine and Hygiene, 95(3), 186-191.

Jenny-Avital, E. R. (2002). Acquired rifampin resistance in AIDS-related TB. AIDS Clinical Care, 14(8), 72-73.

Joshua, O. O., Gospel, T. O., Emeka, U. E., & Chukwukere, E. (2013). Nigerian National TB standard operating procedures manual for Laboratories. National Tuberculosis and Leprosy Control programme (NTBLCP), Ministry of health, Nigeria and American Society for Microbiology, Ist edition, 1-244.

Kolo, I. Bacteriological and drug sensitivity studies on Mycobacteria isolated from tuberculosis patients and their close contacts in ABUTH, Zaria, Nigeria. 1991 (Doctoral dissertation, PhD Thesis, Zaria, Nigeria).

Kumar, V., Abbas, A. K., Fausto, N., and Mitchell, R. N. (2007). Robbins Basic Pathology (8th ed.). pp960 Saunders Elsevier.

Lawn, S. D., & Churchyard, G. (2009). Epidemiology of HIV-associated tuberculosis running head: epidemiology of TB/HIV. Current Opinion in HIV and AIDS, 4(4), 325.

Lawson, L., Yassin, M. A., Abdurrahman, S. T., Parry, C. M., Dacombe, R., Sogaolu, O. M., ... & Cuevas, L. E. (2011). Resistance to first-line tuberculosis drugs in three cities of Nigeria. Tropical Medicine & International Health, 16(8), 974-980.

McGrath, M., Gey van Pittius, N. C., Van Helden, P. D., Warren, R. M., & Warner, D. F. (2014). Mutation rate and the emergence of drug resistance in Mycobacterium tuberculosis. Journal of Antimicrobial Chemotherapy, 69(2), 292-302.

Mizrahi, V., & Andersen, S. J. (1998). DNA repair in Mycobacterium tuberculosis. What have we learnt from the genome sequence?. Molecular microbiology, 29(6), 1331-1339.

Munsiff, S. S., Joseph, S., Ebrahimzadeh, A., & Frieden, T. R. (1997). Rifampin-monoresistant tuberculosis in New York city, 1993–1994. Clinical infectious diseases, 25(6), 1465-1467.

National Tuberculosis and Leprosy Control Programme. Workers Manual. 4th Edition, (2005). Federal Ministry of Health, Department of Public Health, Abuja. pp 338

Siddiqui and Rusch-Gerdes (2006). MGIT Procedure Manual, Geneva, Switzerland: Foundation for Innovative New Diagnostics.

Sun, G., Luo, T., Yang, C., Dong, X., Li, J., Zhu, Y., ... & Gao, Q. (2012). Dynamic population changes in Mycobacterium tuberculosis during acquisition and fixation of drug resistance in patients. The Journal of infectious diseases, 206(11), 1724-1733.

Uzoewulu, N. G., Ibeh, I. N., Lawson, L., Goyal, M., Umenyonu, N., Ofiaeli, R. O., & Okonkwo, R. (2014). Drug resistant Mycobacterium tuberculosis in tertiary hospital south east, Nigeria. Journal of Medical Microbiology & Diagnosis, 3(2), 1.

Van Crevel, R., Ottenhoff, T. H., & Van Der Meer, J. W. (2002). Innate immunity to Mycobacterium tuberculosis. Clinical microbiology reviews, 15(2), 294-309.

Willey, J. M., Sherwood, L., & Woolverton, C. J. (2011). Prescott’s microbiology, 7. New York: McGraw-Hill.

World Health Organization (2006). Global Tuberculosis Control Report, Annex 1 Profiles of high-burden countries.

World Health Organization (2011). The sixteenth global report on tuberculosis.

WHO (2012). Global Tuberculosis Control, Geneva; 14 www.who.int/tb/publications/global_report.

WHO (2014). Global tuberculosis report www.who.int/tb/data. www.raosoft.com/samplesize.html

Downloads

Published

2022-09-06

How to Cite

Terna, L. A. ., Elizabeth, A. U. ., & T Sar, T. . (2022). Role of HIV Infection in Multi-Drug Resistant Tuberculosis in Parts of Benue State, Nigeria. American Journal of Medical Science and Innovation, 1(1), 1–9. Retrieved from https://journals.e-palli.com/home/index.php/ajmsi/article/view/505