Quality, Nutritional Composition and Effect of Various Yoghurt Blends on the Gut by-Products of Ulcerogenic Wistar Rat

Authors

  • Ogunka-Nnoka Cu Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria
  • Enyong Bu Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria
  • Itohan E Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria
  • Asaba E Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria
  • Ozuru P Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria
  • Ogonu F Department of Biochemistry, University of Port Harcourt, Choba, Rivers State Nigeria

DOI:

https://doi.org/10.54536/ajfst.v5i1.6721

Keywords:

Gastric Ulcer, Gut by-Product, Quality, Wistar Rat, Yoghurts

Abstract

The disruption of the gastrointestinal mucosal lining due to excessive secretion of gastric acid results in a gastric ulcer, which presents epigastric pain shortly after meals. The present study investigated the quality and effects of dairy-based (DBY), plant-based (PBY) and dairy/ plant-based (DPBY) yoghurts on gut metabolites in indomethacin-induced Wistar rats. The formulated yoghurt samples were subjected to physicochemical properties, proximate composition, microbiological quality, and sensory attributes, followed by an evaluation of their effect on Gut metabolites. The three yoghurt samples met Codex Alimentarius quality standards for yoghurt, including acceptable pH (3.98 ± 0.05 to 4.56 ± 0.09) and minimum total solids. The microbiological analyses showed product safety, with Total Viable Counts within International Commission on Microbiological Specifications for Foods (ICMSF) limits and high Lactic Acid Bacteria counts, suggesting probiotic capability. Results of proximate analyses showed higher fat content in DBY (6.22%) compared with PBY (2.56%), whereas protein content was greater in the PBY sample (4.02%). Colour was the most preferred attribute, while tanginess was the least favoured based on sensory evaluation. Gut microbial metabolites were significantly (p<0.05) influenced by the various yoghurt blends. Dairy yoghurt promoted elevated caproic (138.85504 ppm) and valeric (73.19835 ppm), plant-based yoghurt increased hexanoic (53.15172 ppm) and isovaleric acids (15.62704 ppm), and the dairy/plant-based blended yoghurt produced high caproic and propanoic acid concentrations. These findings demonstrate that all three yoghurt types are safe, nutritionally adequate, and capable of modulating SCFA production, underscoring their potential as functional dietary components for supporting gastric ulcer management.

Downloads

Download data is not yet available.

References

Adejo, S.O., Kemiie, K.L.& Forwoukeh, V.H., (2024). Production and Quality Assessment of Plant-based Yoghurt from Coconut Milk Fortified with Date Syrup. Asian Food Science Journal, 23, 101-113. http://doi.org10.9734/afsj/2024/v237730

Adejuyitan, J. A., Olanipekun, B. F., & Moyinwin, O. A. (2014). Production and evaluation of a cheese-like product from the blend of soy milk and coconut milk. Archives of Applied Science Study, 6(4), 263-266.

Aidoo, H., Sakyo-Dawson, E., Tano-Debrah K. & Saalia, F. K., (2010). Development and characterization of dehydrated peanut-cowpea milk powder for use as a dairy milk substitute in chocolate manufacture. Food research international, 43 (1), 79-85.

Al-Farsi, M., Alasalva, C., Morris, A., Baron, M., & Shahidi, F., (2005). Compositional and Sensory Characteristics of Three Native Sun-Dried Date (Phoenix dactylifera L.) Varieties Grown in Oman. Journal of Agricultural and Food Chemistry, 53, 7586-7591. http://doi 10.1021/jf050578y

Amadou, N. M., Richard, E., Waingeh, N., Helene, I., Ndombow, Y., & Jules-Roger, K. (2017). Physicochemical and sensory properties of ginger spiced yoghurt. Journal of Nutritional Therapeutics, 6(3), 68–74.

AOAC. (2012). Official methods of analysis (19th ed.). Association of Official Analytical Chemists.

Baburao, V. L., Majumder, S., Kishor, K., Santosh, & Shanta, P. (2019). Studies on physico-chemical quality parameters of skim milk yoghurt fortified with pomegranate juice. International Journal of Food Sciences and Nutrition, 4(1), 49–52.

Bhattacharya, S., Susri Ray Chaudhuri, Chattopadhyay, S., & Bandyopadhyay, S. K. (2007). Healing Properties of Some Indian Medicinal Plants against Indomethacin-Induced Gastric Ulceration of Rats. Journal of Clinical Biochemistry and Nutrition, 41(2), 106–114. https://doi.org/10.3164/jcbn.2007015

El-Kholy, W. M., Soliman, T. N., & Darwish, A. M. G. (2019). Evaluation of date palm pollen (Phoenix dactylifera L.) encapsulation, impact on the nutritional and functional properties of fortified yoghurt. PLOS ONE, 14(10), e0222789. https://doi.org/10.1371/journal.pone.0222789

El-Sayed, A., Aleya, L., & Kamel, M. (2021). Microbiota’s role in health and diseases. Environmental Science and Pollution Study, 28(28), 36967-36983.

Grasso, N., Alonso-Miravalles, L., & O’Mahony, J. A. (2020). Composition, Physicochemical and Sensorial Properties of Commercial Plant-Based Yoghurts. Foods, 9 (3), 252.

Hasnae, B., Youness, T., Nada, B., Yasmine G., Abderrazzak, K., Amina, B., Habiba, B., Khalid, B., Nawal, B.,Barkat, A. & Hassan, A., (2017). Acceptance of sugar reduction in yoghurt among Morrocan populations. Pan African Medical Journal, 28, 310. [doi: 10.11604/pamj.2017.28.310.12257]

Holscher H. D. (2017). Dietary fiber and prebiotics and the gastrointestinal microbiota. Gut microbes, 8(2), 172–184. https://doi.org/10.1080/19490976.2017.1290756

Hussein, A. M. S., Fouad, M. T., Abd El-Aziz, M., Ashour, N. E. N., & Mohamed Mostafa, E. A. (2017). Evaluation of physico-chemical properties of some date varieties and yoghurt made with its syrups. Journal of Biological Sciences, 17, 213-221.

International Commission on Microbiological Specifications for Foods (ICMSF). (2005). Microbial ecology of food commodities (2nd ed., pp. 522–532).

Imhann, F., Bonder, M. J., Vich Vila, A., Fu, J., Mujagic, Z., Vork, L. & Zhernakova, A. (2016). Proton pump inhibitors affect the gut microbiome. Gut, 65(5), 740–748. doi:10.1136/gutjnl-2015-310376

Jackson, M. A., Goodrich, J. K., Maxan, M. E., Freedberg, D. E., Abrams, J. A., Poole, A. C., & Steves, C. J. (2016). Proton pump inhibitors alter the composition of the gut microbiota. Gut, 65(5), 749-756.

Keshavarzian, A., Green, S. J., Engen, P. A., Voigt, R. M., Naqib, A., Forsyth, C. B., Mutlu, E., & Shannon, K. M. (2015). Colonic bacterial composition in Parkinson’s disease. Movement Disorders, 30(10), 1351–1360. https://doi.org/10.1002/mds.26307

Koo, B. S., Fang, Z. F., Kim, H. S., Kim, H., Park, S. J., & Choi, H. K. (2019). Proton pump inhibitors use and risk of fracture: a systematic review and meta-analysis of observational studies. Osteoporosis International, 30(6), 1163-1175.

Le Roy, C.I., Kurilshikov, A., Leeming, E.R. Visconti, A., Bowyer, R.C.E., Menni, C., Falchi, M., Koutnikova, H., Veiga, P., Zhernakova, A., Derrien, M.& Spector, T.D. (2022). Yoghurt consumption is associated with changes in the composition of the human gut microbiome and metabolome. BMC Microbiol 22, 39. https://doi.org/10.1186/s12866-021-02364-2

Leeward, B. O., Alemawor, F., & Deku, G. (2023). Nutritional and sensory evaluation of yoghurt incorporated with unripe false horn plantain (Musa paradisiaca var. “apentu”). International Journal of Food Science, 2023, Article 2221302. https://doi.org/10.1155/2023/2221302

Liu, L., Li, Q., Yang, Y., & Guo, A. (2021). Biological function of short-chain fatty acids and its regulation on intestinal health of poultry. Frontiers in Veterinary Science, 8, 736739.

Lorea, B., Kirjavainen, P., Hekmat, S., & Reid, G. (2007). Anti-inflammatory effects of probiotic yogurt in inflammatory bowel disease patients. Clinical & Experimental Immunology, 149(3), 470–479. https://doi.org/10.1111/j.1365-2249.2007.03434.x

Makanjuola, O.M. (2012). Production and Quality Evaluation of Soy Corn Yoghurt. Advanced Journal of Food Science and Technology, 4, 130-134.

Makinen, O. E., Wanhalinna, V., Zannini, E., & Arendt, E. K. (2016). Foods for special dietary needs: Non-dairy plant-based milk substitutes and fermented dairy-type products. Critical Reviews in Food Science and Nutrition, 56(3), 339–349.

Marco, M., Heeney, D., Binda, S., Cifelli, C., Cotter, P., & Foligné, B. (2017). Health benefits of fermented foods: microbiota and beyond. Current Opinion in Biotechnology, 44(44). https://doi.org/10.1016/j.copbio.2016.11.010

Matin, A., Banik, T., Badsha, M.R., Hossain, A., Haque, M.M.& Ahmad, M., (2018). Microbiological quality analysis of yoghurt in some selected areas of Bangladesh. International Journal of Natural and Social Sciences, 5 (4), 82-86.

Massomian, A., Rashidimehr, A., Mohammadi-Nasrabadi, F., Khoshtinat, K., & Esfarjani, F. (2025). Salt Contents in Fermented Dairy Products: A Strategic Blueprint for Healthier Intake. Food science & nutrition, 13(1), e4762. https://doi.org/10.1002/fsn3.4762

Moldoveanu, S. C., & David, V. (2019). Derivatization methods in GC and GC/MS. In IntechOpen eBooks. https://doi.org/10.5772/intechopen.81954

Nishinari, K., Fang, Y., Guo, S., & Phillips, G. O. (2014). Soy protein: A review on composition, aggregation and emulsification. Food Hydrocolloids, 39, 301–318.

Olson, D. W., & Aryana, K. J. (2022). Probiotic Incorporation into Yogurt and Various Novel Yogurt-Based Products. Applied Sciences, 12(24), 12607. https://doi.org/10.3390/app122412607

Ozer, M. (2009). The roles of product lead-users and product experts in new product evaluation. Study policy, 38(8), 1340-1349.

Sidhu, K., Fengzhi, L., Sharkie, T.P., Ajilouni, S., & Ranadheera, C.S., (2020). Probiotic yoghurt fortified with chickpea flour: Physicochemical properties and probiotic survival during storage and simulated gastrointestinal transit. Foods, 9 (9), 1144.

Tamime, A. Y., & Robinson, R. K. (2007). Tamime and Robinson’s yoghurt: Science and technology. Elsevier. https://doi.org/10.1201/NOEI420044539

Zhou, L., Li, J., Ding, C., Zhou, Y., & Xiao, Z. (2025). Mechanistic Advances in Hypoglycemic Effects of Natural Polysaccharides: Multi-Target Regulation of Glyco-metabolism and Gut Microbiota Crosstalk. Molecules, 30(9), 1980.

Downloads

Published

2026-05-08

How to Cite

Ogunka-Nnoka, . C., Enyong, B., Itohan, E., Asaba , E., Ozuru , P., & Ogonu , F. (2026). Quality, Nutritional Composition and Effect of Various Yoghurt Blends on the Gut by-Products of Ulcerogenic Wistar Rat. American Journal of Food Science and Technology, 5(1), 76-84. https://doi.org/10.54536/ajfst.v5i1.6721

Similar Articles

21-30 of 35

You may also start an advanced similarity search for this article.