The Safety of Leafy Vegetables in Oman
DOI:
https://doi.org/10.54536/ajfst.v3i1.2674Keywords:
Microbiological, Heavy Metal, Contamination, Leafy VegetablesAbstract
Leafy vegetables are potential carriers of foodborne diseases that threaten the community’s well-being. Therefore, monitoring leafy vegetable’s microbial and heavy metal contamination is crucial. This study evaluates the safety of leafy vegetables consumed in Oman by examining pathogenic bacteria such as Escherichia coli, Staphylococci, Salmonella, and Listeria, as well as the heavy metals concentration. Results indicate high levels of microbial contamination in all samples, exceeding the Gulf Standard Organization permissible levels for E. coli and Staphylococci. The highest levels of E. coli were 5.96, 6.08, 6.09, 6.25, and 6.01 log CFU/g in Arugula, Radish, Lettuce, Cabbage, and Spring onion, respectively. While for Staphylococcus the highest values were 5.59, 5.71, 4.33, 5.07, and 4.46 log CFU/g in Arugula, Radish, Lettuce, Cabbage, and Spring onion, respectively. No Salmonella or Listeria colonies were found in any samples even after several days of incubations. The Arsenic and Lead concentrations in all samples were significantly higher than the permissible levels set by FAO/WHO, while Chromium was high in some samples. The concentrations of Copper and Iron in most samples fell below their respective permissible levels, while Zinc was undetectable in all samples. These findings underscore inadequate hygiene, harvesting, storage, and agricultural practices, raising concerns about the health implications of consuming contaminated leafy vegetables.
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References
Abadias, M., Alegre, I., Oliveira, M., Altisent, R., & Viñas, I. (2012). Growth potential of Escherichia coli O157:H7 on fresh-cut fruits (melon and pineapple) and vegetables (carrot and escarole) stored under different conditions. Food Control, 27(1), 37-44. https://doi.org/10.1016/j.foodcont.2012.02.032
Aytac, S. A., Ben, U., Cengiz, C., & Taban, B. M. (2010). Evaluation of Salmonella and Listeria monocytogenes contamination on leafy green vegetables. Journal of Food Agriculture & Environment, 8(2), 275-279. https://doi.org/10.5072/zenodo.36512
Abadias, M., Usall, J., Anguera, M., Solsona, C., & Viñas, I. (2008). Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. International Journal of Food Microbiology, 123(1-2), 121-129. https://doi.org/10.1016/j.ijfoodmicro.2007.12.013
Abatcha, M. G., Tan, P. L., Chuah, L. O., Rusul, G., Chandraprasad, S. R., & Effarizah, M. E. (2020). Evaluation of 3MTM loop-mediated isothermal amplification-based kit and 3MTM ready-to-use plating system for detection of Listeria in naturally contaminated leafy vegetables, chicken, and their related processing environments. Food Science and Biotechnology/Food Science and Biotechnology, 29(8), 1141–1148. https://doi.org/10.1007/s10068-020-00762-2
Babic, I., Watada, A., & Buta, J. (1997). Growth of Listeria monocytogenes Restricted by Native Microorganisms and Other Properties of Fresh-Cut Spinach. Journal of Food Protection, 60(8), 912-917. https://doi.org/10.4315/0362-028x-60.8.912
Balarastaghi, S., Rezaee, R., Hayes, A. W., Yarmohammadi, F., & Karimi, G. (2022). Mechanisms of Arsenic Exposure-Induced Hypertension and Atherosclerosis: an Updated Overview. Biological Trace Element Research, 201(1), 98-113. https://doi.org/10.1007/s12011-022-03153-2
Bawwab, M., Qutob, A., Khatib, M. A., Malassa, H., Shawahna, A., & Qutob, M. (2022). Evaluation of Heavy Metal Concentrations in Soil and Edible Vegetables Grown in Compost from Unknown Sources in Al-Jiftlik, Palestine. Journal of Environmental Protection, 13(01), 112-125. https://doi.org/10.4236/jep.2022.131007
Bingöl, M., Yentür, G., Er, B., & Öktem, A. B. (2010). Determination of some heavy metal levels in soft drinks from Turkey using ICP/OES method. Czech Journal of Food Sciences, 28(3), 213-216. https://doi.org/10.17221/158/2008-cjfs
Choi, Y., Jiyeon, J., Junghoon, P., (2016). Kinetic behavior of pathogenic Escherichia coli and Staphylococcus aureus in fresh vegetables during storage at constant and changing temperature. J. Bioanal. Biostat, 1, 1-6. https://www.avensonline.org/wp-content/uploads/JBABS-2641-8681-01-0004.pdf
Degaga, B., Sebsibe, I., Belete, T., & Asmamaw, A. (2022). Microbial Quality and Safety of Raw Vegetables of Fiche Town, Oromia, Ethiopia. Journal of Environmental and Public Health, 2022, 1-10. https://doi.org/10.1155/2022/2556858
Demi-Rezen, D., & Aksoy, A. (2006). Heavy metal levels in vegetables in turkey are within safe limits for cu, zn, ni and exceeded for cd and Pb. Journal of Food Quality, 29(3), 252-265. https://doi.org/10.1111/j.1745-4557.2006.00072.x
Domergue, F. L., & Védy, J. C. (1992). Mobility of Heavy Metals in Soil Profiles. International Journal of Environmental Analytical Chemistry, 46(1-3), 13-23. https://doi.org/10.1080/03067319208026993
Fröder, H., Martins, C. G., De Souza, K. L. O., Landgraf, M., Franco, B. D., & Destro, M. T. (2007). Minimally Processed Vegetable Salads: Microbial Quality Evaluation. Journal of Food Protection, 70(5), 1277-1280. https://doi.org/10.4315/0362-028x-70.5.1277
Gupta, N., Yadav, K. K., Kumar, V., Prasad, S., Cabral-Pinto, M. M. S., Jeon, B. H., Kumar, S., Abdellattif, M. H., & Alsukaibia, A. K. D. (2022). Investigation of Heavy Metal Accumulation in Vegetables and Health Risk to Humans From Their Consumption. Frontiers in Environmental Science, 10. https://doi.org/10.3389/fenvs.2022.791052
GSO 1016, (2015). Microbiological Criteria For Foodstuffs. Gulf Technical Regulation, GCC Standardization Organization, Approved on 05 November 2015 · Edition 2.
Gezahegn, W. W. (2017). Study of Heavy Metals Accumulation in Leafy Vegetables of Ethiopia. IOSR Journal of Environmental Science, Toxicology and Food Technology, 11(05), 57-68. https://doi.org/10.9790/2402-1105015768
Hardaway, C. J., Sneddon, J., Sneddon, E. J., Kiran, B., Lambert, B. J., McCray, T. C., Bowser, D. Q., & Douvris, C. (2016). Study of selected metal concentrations in sediments by inductively coupled plasma-optical emission spectrometry from a metropolitan and more pristine bayou in Southwest Louisiana, United States. Microchemical Journal, 127, 213-219. https://doi.org/10.1016/j.microc.2016.02.016
Hashemi, S. M. B., Abhari, K., & Khaneghah, A. M. (2019). The combined effects of ultrasound and lactic acid in inactivating microorganisms on fresh radish ( Raphanus raphanistrum subsp. sativus ): Microbiological and quality changes. Food Science & Nutrition, 8(1), 162-169. https://doi.org/10.1002/fsn3.1287
Hanif, R., Zafar, I., Mudassar, I., Shaheena, H., & Masooma, R. (2006). Use of vegetables as nutritional food: role in human health. Journal of Agricultural and Biological Science, 1(1), 18-22. https://www.arpnjournals.com/jabs/research_papers/jabs_0706_03.pdf
Ismail, A., & Sook, F. C. (2003). Determination of vitamin C, β-carotene and riboflavin contents in five green vegetables organically and conventionally grown. Malaysian journal of nutrition, 9(1), 31-39. https://pubmed.ncbi.nlm.nih.gov/22692530/
Jean-Lou Dorne, George EN Kass, Luisa R Bordajandi, Billy Amzal, Ulla Bertelsen, Anna F Castoldi, Claudia Heppner, Mari Eskola, Stefan Fabiansson, Pietro Ferrari, Elena Scaravelli, Eugenia Dogliotti, Peter Fuerst, Alan R Boobis, Philippe Verger (2010). Human risk assessment of heavy metals: principles and applications. Metal Ions in Life Sciences, 8, 27-60. https://pubmed.ncbi.nlm.nih.gov/21473375/
Khalil, R. K. (2016). Effect of abusive storage temperatures on growth and survival of Escherichia coli O157:H7 on leafy salad vegetables in Egypt. Lebensmittel-Wissenschaft + Technologie/Food Science & Technology, 65, 954-962. https://doi.org/10.1016/j.lwt.2015.09.034
Kothe, C. I., Pessoa, J. P., Malheiros, P. S., & Tondo, E. C. (2019). Assessing the growth of Staphylococcus aureus and Escherichia coli on fruits and vegetables. Journal of Infection in Developing Countries, 13(06), 480-486. https://doi.org/10.3855/jidc.10573
Kananke, T., Wansapala, J., & Gunaratne, A. (2014). Heavy Metal Contamination in Green Leafy Vegetables Collected from Selected Market Sites of Piliyandala Area, Colombo District, Sri Lanka. American Journal of Food Science and Technology, 2(5), 139-144. https://doi.org/10.12691/ajfst-2-5-1
Li, W., Pires, S. M., Liu, Z., Ma, X., Liang, J., Jiang, Y., Chen, J., Liang, J., Wang, S., Wang, L., Wang, Y., Meng, C., Huo, X., Lan, Z., Lai, S., Liu, C., Han, H., Liu, J., Fu, P., & Guo, Y. (2020). Surveillance of foodborne disease outbreaks in China, 2003-2017. Food Control, 118, 107359. https://doi.org/10.1016/j.foodcont.2020.107359
Lieu, P. T., Heiskala, M., Peterson, P. A., & Yang, Y. (2001). The roles of iron in health and disease. Molecular Aspects of Medicine, 22(1-2), 1-87. https://doi.org/10.1016/s0098-2997(00)00006-6
Liu, W. H., Zhao, J. Z., Ouyang, Z. Y., Söderlund, L., & Liu, G. H. (2005). Impacts of sewage irrigation on heavy metal distribution and contamination in Beijing, China. Environment International, 31(6), 805-812. https://doi.org/10.1016/j.envint.2005.05.042
Mensah, E., Kyei-Baffour, N., Ofori, E., & Obeng, G. (2009). Influence of Human Activities and Land Use on Heavy Metal Concentrations in Irrigated Vegetables in Ghana and Their Health Implications. In Springer eBooks (pp. 9–14). https://doi.org/10.1007/978-1-4020-9139-1_2
McBride, M. (2003). Toxic metals in sewage sludge-amended soils: has promotion of beneficial use discounted the risks? Advances in Environmental Research, 8(1), 5–19. https://doi.org/10.1016/s1093-0191(02)00141-7
Ma, L., Yang, Z., Kong, Q., & Wang, L. (2017). Extraction and determination of arsenic species in leafy vegetables: Method development and application. Food Chemistry, 217, 524-530. https://doi.org/10.1016/j.foodchem.2016.09.015
Marshall, K. E., Nguyen, T. A., Ablan, M., Nichols, M. C., Robyn, M. P., Sundararaman, P., Whitlock, L., Wise, M. E., & Jhung, M. A. (2020). Investigations of Possible Multistate Outbreaks of Salmonella, Shiga Toxin–Producing Escherichia coli, and Listeria monocytogenes Infections - United States, 2016. Morbidity and Mortality Weekly Report. Surveillance Summaries, 69(6), 1-14. https://doi.org/10.15585/mmwr.ss6906a1
Murphy, C. J., & Oudit, G. Y. (2010). Iron-Overload Cardiomyopathy: Pathophysiology, Diagnosis, and Treatment. Journal of Cardiac Failure, 16(11), 888-900. https://doi.org/10.1016/j.cardfail.2010.05.009
Nava-Ruíz, C., & Méndez-Armenta, M. (2013). Cadmium, Lead, Thallium: Occurrence, Neurotoxicity and Histopathological Changes of the Nervous System. In Environmental chemistry for a sustainable world (pp. 321-349). https://doi.org/10.1007/978-3-319-02387-8_6
Nousiainen, L. L., Joutsen, S., Lunden, J., Hänninen, M. L., & Fredriksson-Ahomaa, M. (2016). Bacterial quality and safety of packaged fresh leafy vegetables at the retail level in Finland. International Journal of Food Microbiology, 232, 73-79. https://doi.org/10.1016/j.ijfoodmicro.2016.05.020
Saiwal, N., Dahiya, M., & Dureja, H. (2019). Nutraceutical Insight into Vegetables and their Potential for Nutrition Mediated Healthcare. Current Nutrition & Food Science, 15(5), 441-453. https://doi.org/10.2174/1573401314666180115151107
Settaluri, V. S., Al-Mamari, K. M. K., Al-Balushi, S. I. M., Al-Risi, M. K. Z., & Ali, M. B. (2015). Review of Biochemical and Nutritional Constituents in Different Green Leafy Vegetables in Oman. Food and Nutrition Sciences, 06(09), 765-769. https://doi.org/10.4236/fns.2015.69079
Souri, M. K., Alipanahi, N. A., & Tohidloo, G. (2016). Heavy metal content of some leafy vegetable crops grown with wastewater in southern suburb of Tehran-Iran. Vegetable Science, 43(2), 156-162. https://doi.org/10.61180/
Sampaio, A. F. S., Silva, M., Dornas, W. C., Costa, D. C., Silva, M. E., Santos, R. C. D., De Lima, W. G., & Pedrosa, M. L. (2014). Iron toxicity mediated by oxidative stress enhances tissue damage in an animal model of diabetes. BioMetals, 27(2), 349-361. https://doi.org/10.1007/s10534-014-9717-8
Sant’Ana, A. S., Landgraf, M., Destro, M. T., & Franco, B. D. (2011). Prevalence and counts of Salmonella spp. in minimally processed vegetables in São Paulo, Brazil. Food Microbiology, 28(6), 1235-1237. https://doi.org/10.1016/j.fm.2011.04.002
Uriu-Adams, J. Y., & Keen, C. L. (2005). Copper, oxidative stress, and human health. Molecular Aspects of Medicine, 26(4-5), 268-298. https://doi.org/10.1016/j.mam.2005.07.015
World Health Organisation (WHO), (1989). Report of 33rd meeting, Joint FAO/WHO Joint Expert Committee on Food Additives, Toxicological evaluation of certain food additives and contaminants No. 24, International Programme on Chemical Safety, WHO, Geneva. https://www.who.int/publications/i/item/9241207760
Xu, W., Chen, H., Huang, Y., & Wu, C. (2013). Decontamination of Escherichia coli O157:H7 on green onions using pulsed light (PL) and PL–surfactant–sanitizer combinations. International Journal of Food Microbiology, 166(1), 102-108. https://doi.org/10.1016/j.ijfoodmicro.2013.06.027
Yafetto, L., Ekloh, E., Sarsah, B., Amenumey, E. K., & Adator, E. H. (2019). Microbiological Contamination of some Fresh Leafy Vegetables Sold in Cape Coast, Ghana. Deleted Journal, 60(2), 11-23. https://doi.org/10.4314/gjs.v60i2.2
Zwolak, A., Sarzyńska, M., Szpyrka, E., & Stawarczyk, K. (2019). Sources of Soil Pollution by Heavy Metals and Their Accumulation in Vegetables: a Review. Water, Air and Soil Pollution/Water, Air & Soil Pollution, 230(7). https://doi.org/10.1007/s11270-019-4221-y
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