Diversity of Mycotoxin-Producing Fungi in Leafy and Fruit Vegetables Sold in Port Harcourt Metropolis, Nigeria
DOI:
https://doi.org/10.54536/ajfst.v3i2.3665Keywords:
Mycotoxin, Leafy and Fruit Vegetables, Diversity, Polymerase Chain Reaction, ITS GeneAbstract
Mycotoxins are secondary metabolites produced by fungi and are capable of causing diseases and even death in both humans and other animals. This study investigated the diversity of mycotoxin-producing fungi from leafy (Telfairia occidentalis and Bassica oleracea) and fruit (Solanum lycopersicum and Cucumis sativus) vegetables sold in Port Harcourt metropolis, Nigeria using molecular techniques. The fungi were isolated from vegetable samples showing signs of disease using standard methods. DNA extraction and Polymerase chain reaction were carried out at the molecular laboratory of Regional Centre of Biotechnology and Bioresources research, University of Port Harcourt. The isolates were screened for the presence of mycotoxigenic genes (nor1 (aflatoxin), tri6 (trichothecene), otanps (Ochratoxin A), fum13 (fumonisin) and ZEA (zearalenone)) using PCR technique. The isolates containing mycotoxins were identified using ITS gene sequences. Ten out of the eighteen isolates were positive for four (nor1, tri6, otanps and fum13) out of the five mycotoxigenic genes screened. The nine isolates included fungi belong to six genera: Meyerozyma, Pithomyces, Fusarium, Trametes, Penicillium and Aspergillus. The ten isolates were classified as Meyerozyma carbbica RCBBR_Sf13, Pithomyces chartarum RCBBR_Sf5, Fusarium falciforme RCBBR_Sf10, Trametes duplexa RCBBR_Sf17, Trametes versicolor RCBBR_Sf2a, Trametes duplexa RCBBR_Mf1, Fusarium longifundum RCBBR_Mf4, Penicillium soosanum RCBBR_Mf7, Aspergillus aflatoxiformans RCBBR_Sf9, Fusarium circinatum RCBBR_Sf3 based on their ITS gene sequences. Their ITS gene sequences have been deposited in GenBank under the accession numbers OR816039-OR816047, with the exception of Fusarium circinatum RCBBR_Sf3 which could not accession. This study has demonstrated that mycotoxin-producing fungi are diverse and widespread in leafy and fruit vegetables sold within Port Harcourt Metropolis. This raises both public health and food security concerns.
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Bennett, J. W., & Klich, M. (2003). Mycotoxins. Clinical Microbiology Reviews, 16(3), 497–516.
Bluhm, B. H., Flaherty, J. E., Cousin, M. A., & Woloshuk, C. P. (2002). Multiplex polymerase chain reaction assay for the differential detection of trichothecene- and fumonisin-producing species of Fusarium in cornmeal. Journal of Food Protection, 65(12), 1955–1960.
Cruz-Perez, P., Buttner, M. P., & Stetzenbach, L. D. (2001). Specific detection of Stachybotrys chartarum in pure culture using quantitative polymerase chain reaction. Molecular and Cellular Probes, 15(3), 129–136.
Eze, V. C., Egwim, E. C., Uadia, P. O., & Ndiokwere, C. L. (2015). Mycotoxin producing fungi in some leafy and fruit vegetables (tomato and cucumber) sold in the markets of Port Harcourt metropolis, Nigeria. Journal of Applied Sciences and Environmental Management, 19(2), 275–283.
Food and Agriculture Organization of the United Nations (FAO). (2004). Worldwide regulations for mycotoxins in food and feed in 2003 (FAO Food and Nutrition Paper No. 81). Food and Agriculture Organization of the United Nations.
Gelderblom, W. C. A., Seier, J. V., Snyman, S. D., & Abel, S. (2016). Fumonisins: A maize problem in perspective. Carcinogenesis, 37(11), 1021–1024.
Golnaraghi, A. R., Pourrahim, R., Ahoonmanesh, A., Zamani-Zadeh, H. R., & Farzadfar, S. (2008). Detection and characterization of a distinct isolate of Tomato Yellow Fruit Ring Virus from potato. Plant Disease, 92(9), 1305–1310.
Grooters, A. M., & Gee, M. K. (2002). Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum. Journal of Veterinary Internal Medicine, 16(2), 147–152.
Hell, K., Cardwell, K. F., Setamou, M., & Poehling, H. M. (2000). The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, West Africa. Journal of Stored Products Research, 36(4), 365–382.
Jones, B., Al-Mughrabi, K., Lopez, D., & Moore, A. (2018). Comparative analysis of DNA concentration in fungal isolates from leafy greens. Plant Pathology Journal, 34(1), 56–67.
Klich, M. A. (2007). Aspergillus flavus: The major producer of aflatoxin. Molecular Plant Pathology, 8(6), 713–722.
Kumar, R., Singh, P., Gupta, M., & Sharma, P. (2021). DNA extraction efficiency in fungal isolates from fruits. Journal of Agricultural Sciences, 29(4), 299–310.
Lawson, A. J., Linton, D., Stanley, J., & Owen, R. J. (1997). Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques. Journal of Applied Microbiology, 83(2), 239–244.
Lee, H., Liu, M., Zhang, S., & Williams, T. (2020). Gel electrophoresis analysis of DNA from fruit vegetables fungal isolates. Molecular Ecology, 22(5), 678–689.
Marasas, W. F. O. (2001). Discovery and occurrence of the fumonisins: A historical perspective. Environmental Health Perspectives, 109(suppl 2), 239–243.
Marasas, W. F. O., Miller, J. D., Riley, R. T., & Visconti, A. (1984). Fumonisins—Occurrence, toxicology, metabolism, and risk assessment. World Health Organization, 2(97), 287–296.
McCormick, S. P., Stanley, A. M., Stover, N. A., & Alexander, N. J. (2011). Trichothecenes: From simple to complex mycotoxins. Toxins, 3(7), 802–814.
O’Brien, E., & Dietrich, D. R. (2005). Ochratoxin A: The continuing enigma. Critical Reviews in Toxicology, 35(1), 33–60.
Ostry, V., Malir, F., Toman, J., & Grosse, Y. (2017). Mycotoxins as human carcinogens—the IARC Monographs classification. Mycotoxin Research, 33(1), 65–73.
Pavón, M. Á., González, I., Martín, R., & García Lacarra, T. (2012). ITS-based detection and quantification of Alternaria spp. in raw and processed vegetables by real-time quantitative PCR. Food Microbiology, 31(1), 37–44.
Pestka, J. J. (2010). Deoxynivalenol: Mechanisms of action, human exposure, and toxicological relevance. Archives of Toxicology, 84, 663–679.
Pfohl-Leszkowicz, A., & Manderville, R. A. (2007). Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans. Molecular Nutrition & Food Research, 51(1), 61–99.
Pitt, J. I., & Hocking, A. D. (2009). Fungi and food spoilage. Springer Science & Business Media.
Riley, R. T., & Merrill, A. H. (2019). Ceramide synthase inhibition by fumonisins: A perfect storm of perturbed sphingolipid metabolism, signaling, and disease. Journal of Lipid Research, 60(7), 1183–1193.
Shakuntala, I., Malik, S. V. S., Barbuddhe, S. B., & Rawool, D. B. (2006). Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Veterinary Microbiology, 117(2-4), 229–234.
Smith, A., Patel, R., & Singh, M. (2019). Extraction and purity of genomic DNA from fungal isolates on leafy vegetables. Journal of Microbial Techniques, 12(3), 345–358.
Sobrova, P., Adam, V., Vasatkova, A., Beklova, M., Zeman, L., & Kizek, R. (2010). Deoxynivalenol and its toxicity. Interdisciplinary Toxicology, 3(3), 94–99.
Tannous, J., Atoui, A., El Khoury, A., Kantar, S., Chdid, N., Oswald, I. P., Puel, O., & Lteif, R. (2015). Development of a real-time PCR assay for Penicillium expansum quantification and patulin estimation in apples. Food Microbiology, 52, 42–49.
Wild, C. P., & Gong, Y. Y. (2010). Mycotoxins and human disease: A largely ignored global health issue. Carcinogenesis, 31(1), 71–82.
Williams, J. H., Phillips, T. D., Jolly, P. E., Stiles, J. K., Jolly, C. M., & Aggarwal, D. (2004). Human aflatoxicosis in developing countries: A review of toxicology, exposure, potential health consequences, and interventions. American Journal of Clinical Nutrition, 80(5), 1106–1122.
Wu, F. (2007). Measuring the economic impacts of Fusarium toxins in animal feeds. Animal Feed Science and Technology, 137(3-4), 363–374.
Zur, G., Hallerman, E. M., Sharf, R., & Kashi, Y. (1999). Development of a polymerase chain reaction-based assay for the detection of Alternaria fungal contamination in food products. Journal of Food Protection, 62(11), 1191–1197.
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