Harnessing Epigenetic Modifications for Targeted Trait Manipulation in Pakistani Wheat
DOI:
https://doi.org/10.54536/ijpf.v1i1.2227Keywords:
Epigenetics, Triticum, Disease Resistance, Crop Improvement, AgronomyAbstract
This study explores the potential of harnessing epigenetic modifications for targeted trait manipulation in Pakistani wheat varieties. Epigenetic regulation is pivotal in modulating gene expression patterns and can be leveraged to enhance agronomic traits. A diverse panel of Pakistani wheat varieties was subjected to comprehensive epigenetic profiling, encompassing DNA methylation and histone modification analyses. CRISPR-based epigenetic editing targeted specific genes associated with disease resistance, yield, and nutritional content. Field trials were conducted to evaluate the agronomic performance of edited lines. Epigenetic profiling revealed distinct DNA methylation and histone modification patterns across the selected wheat varieties. Targeted epigenetic editing amplified disease resistance, enhanced yield potential, and enriched nutritional content. Disease incidence and severity were significantly reduced in edited lines. Additionally, increased tiller number, larger grain size, and elevated levels of essential nutrients were observed. This research demonstrates the feasibility and efficacy of employing epigenetic modifications for precise trait manipulation in Pakistani wheat. The study provides valuable insights into the regulatory mechanisms underlying disease resistance, yield, and nutritional traits. Collaborative efforts among geneticists, molecular biologists, and plant breeders are essential in further refining and implementing these innovative breeding techniques. The findings pave the way for developing resilient and nutritionally enriched wheat varieties, contributing to global food security and agricultural sustainability.
References
Bannister, A. J., & Kouzarides, T. (2011). Regulation of chromatin by histone modifications. Cell research, 21(3), 381-395.
Baulcombe, D. C., & Dean, C. (2014). Epigenetic regulation in plant responses to the environment. Cold Spring Harbor perspectives in biology, 6(9), a019471.
Bouis, H. E., & Saltzman, A. (2017). Improving nutrition through biofortification: A review of evidence from HarvestPlus, 2003 through 2016. Global Food Security, 12, 49-58. https://doi.org/https://doi.org/10.1016/j.gfs.2017.01.009
Demetriou, K., Kapazoglou, A., Tondelli, A., Francia, E., Stanca, M. A., Bladenopoulos, K., & Tsaftaris, A. S. (2009). Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant-specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment. Physiol Plant, 136(3), 358-368. https://doi.org/10.1111/j.1399-3054.2009.01236.x
Dowen, R. H., Pelizzola, M., Schmitz, R. J., Lister, R., Dowen, J. M., Nery, J. R., Dixon, J. E., & Ecker, J. R. (2012). Widespread dynamic DNA methylation in response to biotic stress. Proceedings of the National Academy of Sciences, 109(32), E2183-E2191.
Dowen, R. H., Pelizzola, M., Schmitz, R. J., Lister, R., Dowen, J. M., Nery, J. R., Dixon, J. E., & Ecker, J. R. (2012). Widespread dynamic DNA methylation in response to biotic stress. Proc Natl Acad Sci U S A, 109(32), E2183-2191. https://doi.org/10.1073/pnas.1209329109
Feng, S., Jacobsen, S. E., & Reik, W. (2010). Epigenetic reprogramming in plant and animal development. Science, 330(6004), 622-627.
Karanthamalai, J., Chodon, A., Chauhan, S., & Pandi, G. (2020). DNA N(6)-Methyladenine Modification in Plant Genomes-A Glimpse into Emerging Epigenetic Code. Plants (Basel), 9(2). https://doi.org/10.3390/plants9020247
Kouzarides, T. (2007). Chromatin modifications and their function. Cell, 128(4), 693-705.
Lämke, J., & Bäurle, I. (2017). Epigenetic and chromatin-based mechanisms in environmental stress adaptation and stress memory in plants. Genome biology, 18(1), 1-11.
Li, S., Zhang, C., Li, J., Yan, L., Wang, N., & Xia, L. (2021). Present and future prospects for wheat improvement through genome editing and advanced technologies. Plant Communications, 2(4).
Liu, Y., Koornneef, M., & Soppe, W. J. (2007). The absence of histone H2B monoubiquitination in the Arabidopsis hub1 (rdo4) mutant reveals a role for chromatin remodeling in seed dormancy. Plant Cell, 19(2), 433-444. https://doi.org/10.1105/tpc.106.049221
Pakistan Economic Survey. (2021). http://www.finance.gov.pk/survey/chapters_21/02-Agriculture.pdf
Pienaar, A. (2020). An exploration of commercial and subsistence farmers climate change adaption strategies in the Ditsobotla-local municipality, North-West Province, South Africa North-West University (South Africa)].
Ramírez-González, R., Borrill, P., Lang, D., Harrington, S., Brinton, J., Venturini, L., Davey, M., Jacobs, J., Van Ex, F., & Pasha, A. (2018). The transcriptional landscape of polyploid wheat. Science, 361(6403), eaar6089.
Sun, L., Miao, X., Cui, J., Deng, J., Wang, X., Wang, Y., Zhang, Y., Gao, S., & Yang, K. (2018). Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across different salt stress in Maize (Zea mays L.). Euphytica, 214, 1-15.
Xu, R.-F., Li, H., Qin, R.-Y., Li, J., Qiu, C.-H., Yang, Y.-C., Ma, H., Li, L., Wei, P.-C., & Yang, J.-B. (2015). Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system. Scientific reports, 5(1), 11491.
Zhang, H., Lang, Z., & Zhu, J.-K. (2018). Dynamics and function of DNA methylation in plants. Nature reviews Molecular cell biology, 19(8), 489-506.
Zulfiqar, F., & Thapa, G. B. (2017). Agricultural sustainability assessment at provincial level in Pakistan. Land use policy, 68, 492-502.
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Copyright (c) 2023 Moiz Ahmed, Hamza Tariq, Fizza Haroon, Simrah Malik, Muhammad Shaheer Dar, Faryal Shakil Solangi, Syeda Hafsa Aamir
This work is licensed under a Creative Commons Attribution 4.0 International License.
