Antibacterial Activity of Cassava Manihot Esculenta Leaves Extract Against Escherichia coli

Authors

  • Angel Lhi Dela Cruz Notre Dame of Midsayap College, Midsayap, Cotabato, Philippines.
  • Neil John L. Buendia Notre Dame of Midsayap College, Midsayap, Cotabato, Philippines.
  • Rikka Bianca J. Condes Notre Dame of Midsayap College, Midsayap, Cotabato, Philippines.

DOI:

https://doi.org/10.54536/ajec.v1i2.484

Keywords:

Escherichia coli, Manihot esculenta, Leaves Extract, Antibacterial, Cassava

Abstract

The purpose of this research is to determine the antibacterial activity of Cassava (Manihot esculenta) leaf extract against Escherichia coli. The experiment involved the determination of plants, extraction using the maceration method, and antibacterial testing of the fresh and dried cassava (Manihot esculenta) leave extracted with concentrations of 5%, 10%, 20%, and 30% ethanol against the bacterial growth of (Escherichia coli) and culture media using Merck Nutrient Agar. A caliper was used to measure the size of (Escherichia coli) growth in millimeters. The results showed that extracts of fresh and dried cassava (Manihot esculenta) leaves extract with ethanol concentrations of 5%, 10%, 20%, and 30% have antibacterial activity against Escherichia coli. This study examined the efficacy of fresh and dried cassava (Manihot esculenta) leaves extract against (Escherichia coli) in the 18th and 24th hours. The mean of fresh cassava (Manihot esculenta) leaves extract is 4.4, which differs considerably from the mean of dried Cassava (Manihot esculenta) leaves extract, which is 3.9. The fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli) in the 18th hour, according to the findings of this investigation. Furthermore, at the 24th hour, the fresh cassava (Manihot esculenta) leaves extract was the most potent therapy against (Escherichia coli). Fresh cassava (Manihot esculenta) leaves extract has a mean of 4.4, which differs significantly from dried (Manihot esculenta) leaves extract, which has a mean of 3.9. According to the findings of this study's testing, the 20 percent concentration of fresh cassava (Manihot esculenta) leaves extract with an average of 5.75 was the most active treatment against (Escherichia coli). It has been proposed that cassava (Manihot esculenta) leaves could be turned into standardized antibacterial herbal. Future research should investigate the effects of cassava (Manihot esculenta) leaves used as an antibacterial agent on human health.

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Published

2022-08-18

How to Cite

Dela Cruz, A. L., L. Buendia, N. J. ., & J. Condes, R. B. . (2022). Antibacterial Activity of Cassava Manihot Esculenta Leaves Extract Against Escherichia coli. American Journal of Environment and Climate, 1(2), 23–30. https://doi.org/10.54536/ajec.v1i2.484

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