Investigating how Noscapine Influences Activity and Seizures Caused by the Intake of Alkaloids in Mice
DOI:
https://doi.org/10.54536/ajcp.v4i2.5737Keywords:
Hydromorphine, Noscapine, Seizures, Vital ActivitiesAbstract
This study investigates the effects of noscapine on seizure-like activity and vital functions in mice exposed to alkaloid dependence, with the aim of elucidating its potential therapeutic properties. Specifically, the research explores whether noscapine can mitigate or modulate seizure events triggered by alkaloid exposure, thereby contributing to a broader understanding of its pharmacological profile. Findings from this study may provide valuable insights for the development of novel therapeutic strategies for the management of seizures and other neurological disorders associated with alkaloid consumption. A total of forty newborn healthy mice were randomly selected for the experiment. To induce alkaloid dependence, hydromorphine was administered subcutaneously at doses of 2, 4, 8, 16, and 32 mg/kg (0.1 cc) once daily from postnatal day 14 to day 17. On postnatal days 18 and 19, brain sections were prepared, and epileptiform activities were induced through cerebrospinal fluid perfusion with reduced magnesium levels. The experimental design evaluated the effects of hydromorphine at concentrations of 10, 100, and 1000 μM, as well as noscapine at 10 μM. Quantitative parameters including the number, onset latency, and amplitude of seizure-like activities were assessed as indicators of drug effects. The results demonstrated that hydromorphine exhibits a concentration-dependent, triphasic effect on seizure-like activity during the lactation period: low and high concentrations attenuated seizure activity, whereas moderate concentrations enhanced it. By contrast, noscapine exerted a significant anticonvulsant effect, supporting its potential as a modulator of alkaloid-induced seizures.
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Copyright (c) 2025 Noorullah Neekbin, Mohammad Yonus Sahil, Mahdi Mohammadi, Abdul Qayom Rajabi
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