Preliminary Evaluation for Anti-Mitotic Activities of Aqueous Piper sarmentosum Roxb.Leaf Extract in Allium fistulosum Root Tip Cells
DOI:
https://doi.org/10.54536/ajlsi.v5i1.6446Keywords:
Herbaceous, Mitotic Aberrations, Mitotic Index, Plant ModelsAbstract
Piper sarmentosum Roxb. is an herbaceous plant recognized for its pungent and aromatic smell. Numerous reports of the active phytochemicals, and bioactive properties were documented, however, studies specifically to the anti-mitotic activities of P. sarmentosum is yet limited due to the lack of direct information, comparative studies, and the involvement of plant models. The study aimed to perform a preliminary evaluation of the anti-mitotic activities of the aqueous leaf extract of Piper sarmentosum Roxb. in Allium fistulosum root tip cells. Roots of A. fistulosum were exposed to varying treatment concentrations P. sarmentosum aqueous extract (10%, 30%, 50%), distilled water served as the control group (0%). All root samples were processed and observed under a microscope. Anti-mitotic activities were evaluated based on the mitotic index and manifestation of mitotic abberations. The aqueous leaf extract of P. sarmentosum exhibited decreasing mitotic index relative to the increasing treatment concentrations (0% = 66.00%, 10% = 34.00%, 30% = 16.33%, 50% = 11.00%; p-value = 0.001). Manifestations of mitotic aberrations in Allium fistulosum root cells include chromosome adhesion, c-mitosis, chromosome bridging, chromosomal breakages, and binucleated cell. The results of the study provide additional information, and the need for validation in a larger sample is evident.
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