Optimizing Synergistic Metabolism in Xenobiotic Biodegradation: Engineering a Pseudomonas Putida Based Microbial Consortium

Authors

  • Moiz Ahmed Department of Biotechnology, Shaheed Zulfiqar Ali Bhutto Institute of Science and Technology (SZABIST), Karachi, 75600, Pakistan
  • Hamza Tariq Department of Biotechnology, Shaheed Zulfiqar Ali Bhutto Institute of Science and Technology (SZABIST), Karachi, 75600, Pakistan
  • Fizza Haroon Department of Biotechnology, Shaheed Zulfiqar Ali Bhutto Institute of Science and Technology (SZABIST), Karachi, 75600, Pakistan
  • Tanzila Ejaz Faculty of Science, Department of Food Science and Technology, Jinnah University for Women, Nazimabad, Karachi, Sindh, Pakistan, 74600
  • Maheen Tajwar Department of Biosciences, Forman Christian College, University, Lahore, 54600, Pakistan

DOI:

https://doi.org/10.54536/ajlsi.v3i1.2236

Keywords:

Consortium, Biodegradation, Pseudomonas, Metabolomics, Synergy

Abstract

This study focuses on the engineering of a microbial consortium, incorporating Pseudomonas putida and selected members, to enhance synergistic metabolism for xenobiotic biodegradation. The aim is to explore the potential of microbial interactions in environmental remediation. The engineered consortium underwent iterative optimization to fine-tune microbial ratios. Metabolomic and transcriptomic analyses were employed to investigate metabolic interactions within the consortium. Xenobiotic biodegradation efficiency was evaluated through controlled laboratory experiments. The engineered microbial consortium exhibited a significant synergistic effect, demonstrating enhanced biodegradation capabilities compared to individual monocultures. The iterative optimization process led to a substantial increase in biodegradation efficiency. Metabolomic and transcriptomic analyses provided valuable insights into the metabolic interactions within the consortium. The study successfully engineered a microbial consortium for efficient xenobiotic biodegradation, showcasing the potential of microbial interactions in environmental remediation. The optimized consortium composition and advanced analytical techniques offer promising avenues for sustainable bioremediation practices. Further research is warranted to explore broader applications and address potential scalability challenges.

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References

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Published

2023-12-27

How to Cite

Ahmed, M., Tariq, H., Haroon, F., Ejaz, T., & Tajwar, M. (2023). Optimizing Synergistic Metabolism in Xenobiotic Biodegradation: Engineering a Pseudomonas Putida Based Microbial Consortium. American Journal of Life Science and Innovation, 3(1), 1–7. https://doi.org/10.54536/ajlsi.v3i1.2236

Issue

Vol. 3 No. 1 (2024): American Journal of Life Science and Innovation

Section

Research Articles

License

Copyright (c) 2023 Moiz Ahmed, Hamza Tariq, Fizza Haroon, Tanzila Ejaz, Maheen Tajwar

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

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