Multiplication of Oyster Mushroom Mother Spawn (Pleurotus Ostreatus L.) Using Different Boiling Periods of Sorghum


  • Ambos A. Allysa Joie Mindanao State University (Main) Marawi City, Lanao del Sur, Philippines.
  • Ambos L. Alberto Mindanao State University (Main) Marawi City, Lanao del Sur, Philippines



Boiling Periods, Mother Spawn, Oyster Mushroom, Sorghum


The study was focused on the multiplication of oyster mushroom mother spawn using different boiling periods of sorghum. It was conducted at the Mushroom Laboratory of the Department of Plant Science – College of Agriculture, Mindanao State University Main Campus, Marawi City, from March 31, 2022, to April 21, 2022. The length of mycelia of each treatment is measured every five (5) days for the expansion of mycelia inside the bottles of sorghum. The ANOVA was the statistical tool that was used in analyzing the data. The results revealed that (1) there is a significant difference in the number of days from inoculation to colonization of substrate in every treatment; (2) Treatment 1 has the slowest mycelial growth in the bottle; (3) Treatment 2 has the fastest mycelial growth in the bottle; (4) there is a level of 10% significant difference in the length of mycelial growth in every treatment on the third data gathering; (5) Treatment 1 (fifteen-minute boiling period) has the slowest colonization of the sorghum as mother spawn substrate in the bottle, (6) Treatment 2 (thirty-minute boiling period) has the fastest colonization of the sorghum as mother spawn substrate in the bottle, and (7) Treatment 2 (thirty-minute boiling period) has the best boiling period of the substrate for mother spawn multiplication.


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How to Cite

Joie, A. A. A., & Ambos , L. A. (2022). Multiplication of Oyster Mushroom Mother Spawn (Pleurotus Ostreatus L.) Using Different Boiling Periods of Sorghum. American Journal of Agricultural Science, Engineering, and Technology, 6(3), 78–82.