The Role of Biotechnology in Biodiversity Conservation and Ecological Restoration: A Review

Authors

  • Oluwaseyi Joseph Olukayode Biology institute, Tomsk State University, 36 Lenin Avenue, Tomsk, Tomsk Oblast, Russia
  • Oghenerabome Okpro Biology institute, Tomsk State University, 36 Lenin Avenue, Tomsk, Tomsk Oblast, Russia
  • Sokari Tomipapamieari West Biology institute, Tomsk State University, 36 Lenin Avenue, Tomsk, Tomsk Oblast, Russia
  • Zaaya Sylvia Aver School of Earth Sciences & Engineering, Division of Geology, Ecology and Environmental Engineering, Tomsk Polytechnic University, Lenin Avenue, 30 Tomsk, Tomsk Oblast, Russia

DOI:

https://doi.org/10.54536/ajlsi.v5i2.8384

Keywords:

Biobanking, Biodiversity, Conservation Biotechnology, Conservation Genomics, Ecological Restoration, Environmental DNA, Genome Editing, Microbiome

Abstract

More recently, biotechnology is increasingly adopted alongside conventional approaches to biodiversity conservation and ecosystem restoration. In this study, we examined 60 articles published from year 1998-2024 by grouping the literature into six areas: conservation genomics and genetic rescue, environmental DNA (eDNA), biobanking and reproductive biotechnology, synthetic biology and genome editing, microbiome-based restoration and the governance of genetic data, risks and benefit sharing. The studies reviewed show that genomic approaches can provide information on genetic diversity, inbreeding, population structure, adaptation and whether interventions such as translocation or genetic rescue may be appropriate. eDNA has also expanded the options available for biodiversity monitoring because organisms can be detected from water, soil and air samples without necessarily being captured or directly observed. Studies on cryopreservation, assisted reproduction and stem-cell technologies further demonstrate ways of retaining genetic material that may be difficult to conserve through captive breeding alone. However, the evidence was less straightforward for genome editing and gene drives. Although these technologies may allow highly targeted interventions, the examined studies repeatedly raise questions about unintended ecological effects, reversibility, regulation and public acceptance. Microbiome-based approaches are also becoming promising, particularly where microbial communities influence establishment, stress tolerance or recovery, but their outcomes appear to depend strongly on the species and environmental conditions involved. Hence, the studies reviewed suggest that biotechnology should not be treated as a replacement for habitat protection or ecological restoration. Its value lies mainly in extending the range of tools available to conservation practitioners. Effective use will require appropriate sampling, reliable genetic and ecological data, field validation, long-term monitoring and careful consideration of risk, access to genetic information and benefit sharing.

Downloads

Download data is not yet available.

References

Allendorf, F. W., Hohenlohe, P. A., & Luikart, G. (2010). Genomics and the future of conservation genetics. Nature Reviews Genetics, 11(10), 697–709. https://doi.org/10.1038/nrg2844

Aprahamian, A. M., Lulow, M. E., Major, M. R., Balazs, K. R., Treseder, K. K., & Maltz, M. R. (2016). Arbuscular mycorrhizal inoculation in coastal sage scrub restoration. Botany, 94(6), 493–499. https://doi.org/10.1139/cjb-2015-0226

Bohmann, K., Evans, A., Gilbert, M. T. P., Carvalho, G. R., Creer, S., Knapp, M., Yu, D. W., & de Bruyn, M. (2014). Environmental DNA for wildlife biology and biodiversity monitoring. Trends in Ecology & Evolution, 29(6), 358–367. https://doi.org/10.1016/j.tree.2014.04.003

Breed, M. F., Harrison, P. A., Blyth, C., Byrne, M., Gaget, V., Gellie, N. J. C., Groom, S. V. C., Hodgson, R., Mills, J. G., Prowse, T. A. A., Steane, D. A., & Mohr, J. J. (2019). The potential of genomics for restoring ecosystems and biodiversity. Nature Reviews Genetics, 20(10), 615–628. https://doi.org/10.1038/s41576-019-0152-0

Brink, M., & van Hintum, T. (2022). Practical consequences of digital sequence information (DSI) definitions and access and benefit-sharing scenarios from a plant genebank’s perspective. Plants, People, Planet, 4(1), 23–32. https://doi.org/10.1002/ppp3.10201

Buerger, P., Alvarez-Roa, C., Coppin, C. W., Pearce, S. L., Chakravarti, L. J., Oakeshott, J. G., Edwards, O. R., & van Oppen, M. J. H. (2020). Heat-evolved microalgal symbionts increase coral bleaching tolerance. Science Advances, 6(20), eaba2498. https://doi.org/10.1126/sciadv.aba2498

Burger, I. J., Chen, L. D., Lampert, S. S., Kouba, C. K., Barber, D., Smith, D., Cobos, C., & Kouba, A. J. (2023). Applying sperm collection and cryopreservation protocols developed in a model amphibian to three threatened anuran species targeted for biobanking management. Biological Conservation, 277, 109850. https://doi.org/10.1016/j.biocon.2022.109850

Carballar-Lejarazú, R., Ogaugwu, C., Tushar, T., Kelsey, A., Pham, T. B., Murphy, J., Schmidt, H., Lee, Y., Lanzaro, G. C., & James, A. A. (2020). Next-generation gene drive for population modification of the malaria vector mosquito, Anopheles gambiae. Proceedings of the National Academy of Sciences, 117(37), 22805–22814. https://doi.org/10.1073/pnas.2010214117

Clare, E. L., Economou, C. K., Bennett, F. J., Dyer, C. E., Adams, K., McRobie, B., Drinkwater, R., & Littlefair, J. E. (2022). Measuring biodiversity from DNA in the air. Current Biology, 32(3), 693–700.e5. https://doi.org/10.1016/j.cub.2021.11.064

Comizzoli, P. (2015). Biobanking efforts and new advances in male fertility preservation for rare and endangered species. Asian Journal of Andrology, 17(4), 640. https://doi.org/10.4103/1008-682X.153849

DeFilippo, L. B., McManus, L. C., Schindler, D. E., Pinsky, M. L., Colton, M. A., Fox, H. E., Tekwa, E., Palumbi, S. R., Essington, T. E., & Webster, M. M. (2022). Assessing the potential for demographic restoration and assisted evolution to build climate resilience in coral reefs. Ecological Applications, 32(7), e2650. https://doi.org/10.1002/eap.2650

Deiner, K., Fronhofer, E. A., Mächler, E., Walser, J. C., & Altermatt, F. (2016). Environmental DNA reveals that rivers are conveyor belts of biodiversity information. Nature Communications, 7, 12544. https://doi.org/10.1038/ncomms12544

Deiner, K., Bik, H. M., Mächler, E., Seymour, M., Lacoursière-Roussel, A., Altermatt, F., Creer, S., Bista, I., Lodge, D. M., de Vere, N., Pfrender, M. E., & Bernatchez, L. (2017). Environmental DNA metabarcoding: Transforming how we survey animal and plant communities. Molecular Ecology, 26(21), 5872–5895. https://doi.org/10.1111/mec.14350

Doering, T., Wall, M., Putchim, L., Rattanawongwan, T., Schroeder, R., Hentschel, U., & Roik, A. (2021). Towards enhancing coral heat tolerance: A “microbiome transplantation” treatment using inoculations of homogenized coral tissues. Microbiome, 9(1), 102. https://doi.org/10.1186/s40168-021-01053-6

Esvelt, K. M., Smidler, A. L., Catteruccia, F., & Church, G. M. (2014). Concerning RNA-guided gene drives for the alteration of wild populations. eLife, 3, e03401. https://doi.org/10.7554/elife.03401

Exposito-Alonso, M., Booker, T. R., Czech, L., Gillespie, L., Hateley, S., Kyriazis, C. C., Lang, P. L. M., Leventhal, L., Nogues-Bravo, D., Pagowski, V., Ruffley, M., Spence, J. P., Toro Arana, S. E., Weiß, C. L., & Zess, E. (2022). Genetic diversity loss in the Anthropocene. Science, 3776613, 1431–1435. https://doi.org/10.1126/science.abn5642

Ficetola, G. F., Miaud, C., Pompanon, F., & Taberlet, P. 2008). Species detection using environmental DNA from water samples. Biology Letters, 44, 423–425. https://doi.org/10.1098/rsbl.2008.0118

Formenti, G., Theissinger, K., Fernandes, C., Bista, I., Bombarely, A., Bleidorn, C., ... & Zammit, G. (2022). The era of reference genomes in conservation genomics. Trends in ecology & evolution, 37(3), 197-202. https://doi.org/10.1016/j.tree.2021.11.008

Frankham, R. (2015). Genetic rescue of small inbred populations: Meta-analysis reveals large and consistent benefits of gene flow. Molecular Ecology, 24(11), 2610–2618. https://doi.org/10.1111/mec.13139

Friedrich Ben-Nun, I., Montague, S. C., Houck, M. L., Tran, H. T., Garitaonandia, I., Leonardo, T. R., Wang, Y. C., Charter, S. J., Laurent, L. C., Ryder, O. A., & Loring, J. F. (2011). Induced pluripotent stem cells from highly endangered species. Nature Methods, 8(10), 829–831. https://doi.org/10.1038/nmeth.1706

Funk, W. C., McKay, J. K., Hohenlohe, P. A., & Allendorf, F. W. (2012). Harnessing genomics for delineating conservation units. Trends in Ecology & Evolution, 27(9), 489–496. https://doi.org/10.1016/j.tree.2012.05.012

Gantz, V. M., & Bier, E. (2015). The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations. Science, 348(6233), 442–444. https://doi.org/10.1126/science.aaa5945

Grunwald, H. A., Gantz, V. M., Poplawski, G., Xu, X. R. S., Bier, E., & Cooper, K. L. (2019). Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline. Nature, 566(7742), 105–109. https://doi.org/10.1038/s41586-019-0875-2

Halewood, M., Bagley, M. A., Wyss, M., & Scholz, A. H. (2023). New benefit-sharing principles for digital sequence information. Science, 382(6670), 520–522. https://doi.org/10.1126/science.adj1331

Hammond, A., Galizi, R., Kyrou, K., Simoni, A., Siniscalchi, C., Katsanos, D., Gribble, M., Baker, D., Marois, E., Russell, S., Burt, A., Windbichler, N., Crisanti, A., & Nolan, T. (2016). A CRISPR–Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae. Nature Biotechnology, 34(1), 78–83. https://doi.org/10.1038/nbt.3439

Harris, J. (2009). Soil microbial communities and restoration ecology: Facilitators or followers? Science, 325(5940), 573–574. https://doi.org/10.1126/science.1172975

Hildebrandt, T. B., Hermes, R., Colleoni, S., Diecke, S., Holtze, S., Renfree, M. B., Stejskal, J., Hayashi, K., Drukker, M., Loi, P., Göritz, F., Lazzari, G., & Galli, C. (2018). Embryos and embryonic stem cells from the white rhinoceros. Nature Communications, 91, 2589. https://doi.org/10.1038/s41467-018-04959-2

Hoffmann, A., Griffin, P., Dillon, S., Catullo, R., Rane, R., Byrne, M., Jordan, R., Oakeshott, J., Weeks, A., Joseph, L., Lockhart, P., Borevitz, J., & Sgrò, C. (2015). A framework for incorporating evolutionary genomics into biodiversity conservation and management. Climate Change Responses, 2(1), 1. https://doi.org/10.1186/s40665-014-0009-x

Hohenlohe, P. A., Funk, W. C., & Rajora, O. P. (2021). Population genomics for wildlife conservation and management. Molecular Ecology, 30(1), 62–82. https://doi.org/10.1111/mec.15720

Jackson, H. A., Percival‐Alwyn, L., Ryan, C., Albeshr, M. F., Venturi, L., Morales, H. E., Mathers, T. C., Cocker, J., Speak, S. A., Accinelli, G. G., Barker, T., Heavens, D., Willman, F., Dawson, D., Ward, L., Tatayah, V., Zuël, N., Young, R., Concannon, L., … Van Oosterhout, C. (2022). Genomic erosion in a demographically recovered bird species during conservation rescue. Conservation Biology, 36(4), e13918. https://doi.org/10.1111/cobi.13918

Jerde, C. L., Mahon, A. R., Chadderton, W. L., & Lodge, D. M. (2011). “sight‐unseen” detection of rare aquatic species using environmental DNA. Conservation Letters, 42, 150–157. https://doi.org/10.1111/j.1755-263x.2010.00158.x

Johnson, W. E., Onorato, D. P., Roelke, M. E., Land, E. D., Cunningham, M., Belden, R. C., McBride, R., Jansen, D., Lotz, M., Shindle, D., Howard, J., Wildt, D. E., Penfold, L. M., Hostetler, J. A., Oli, M. K., & O’Brien, S. J. (2010). Genetic restoration of the Florida panther. Science, 329(5999), 1641–1645. https://doi.org/10.1126/science.1192891

Klünker, I., & Richter, H. (2022). Digital sequence information between benefit-sharing and open data. Journal of Law and the Biosciences, 92, lsac035. https://doi.org/10.1093/jlb/lsac035

Kyrou, K., Hammond, A. M., Galizi, R., Kranjc, N., Burt, A., Beaghton, A. K., Nolan, T., & Crisanti, A. (2018). A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes. Nature Biotechnology, 3611, 1062–1066. https://doi.org/10.1038/nbt.4245

Lawson, C., & Rourke, M. (2020). Digital sequence information as a marine genetic resource under the proposed UNCLOS legally binding instrument. Marine Policy, 122, 103878. https://doi.org/10.1016/j.marpol.2020.103878

Li, D. Z., & Pritchard, H. W. (2009). The science and economics of ex situ plant conservation. Trends in Plant Science, 1411, 614–621. https://doi.org/10.1016/j.tplants.2009.09.005

Noble, C., Adlam, B., Church, G. M., Esvelt, K. M., & Nowak, M. A. (2018). Current CRISPR gene drive systems are likely to be highly invasive in wild populations. eLife, 7, e33423. https://doi.org/10.7554/elife.33423

Pence, V. C. (2013). In vitro methods and the challenge of exceptional species for target 8 of the Global Strategy for Plant Conservation. Annals of the Missouri Botanical Garden, 992, 214–220. https://doi.org/10.3417/2011112

Piaggio, A. J., Segelbacher, G., Seddon, P. J., Alphey, L., Bennett, E. L., Carlson, R. H., Friedman, R. M., Kanavy, D., Phelan, R., Redford, K. H., Rosales, M., Slobodian, L., & Wheeler, K. (2017). Is it time for synthetic biodiversity conservation? Trends in Ecology & Evolution, 322, 97–107. https://doi.org/10.1016/j.tree.2016.10.016

Ralls, K., Sunnucks, P., Lacy, R. C., & Frankham, R. (2020). Genetic rescue: a critique of the evidence supports maximizing genetic diversity rather than minimizing the introduction of putatively harmful genetic variation. Biological Conservation, 251, 108784. https://doi.org/10.1016/j.biocon.2020.108784

Redford, K. H., Adams, W., Carlson, R., Mace, G. M., & Ceccarelli, B. (2014). Synthetic biology and the conservation of biodiversity. Oryx, 483, 330–336. https://doi.org/10.1017/s0030605314000040

Robinson, J. A., Räikkönen, J., Vucetich, L. M., Vucetich, J. A., Peterson, R. O., Lohmueller, K. E., & Wayne, R. K. (2019). Genomic signatures of extensive inbreeding in Isle Royale wolves, a population on the threshold of extinction. Science Advances, 5(5), eaau0757. https://doi.org/10.1126/sciadv.aau0757

Rosado, P. M., Leite, D. C. A., Duarte, G. A. S., Chaloub, R. M., Jospin, G., Nunes da Rocha, U., Saraiva, J. P., Dini-Andreote, F., Eisen, J. A., Bourne, D. G., & Peixoto, R. S. (2019). Marine probiotics: Increasing coral resistance to bleaching through microbiome manipulation. The ISME Journal, 13(4), 921–936. https://doi.org/10.1038/s41396-018-0323-6

Sales, N. G., McKenzie, M. B., Drake, J., Harper, L. R., Browett, S. S., Coscia, I., Wangensteen, O. S., Baillie, C., Bryce, E., Dawson, D. A., Ochu, E., Hänfling, B., Lawson Handley, L., Mariani, S., Lambin, X., Sutherland, C., & McDevitt, A. D. (2020). Fishing for mammals: landscape‐level monitoring of terrestrial and semi‐aquatic communities using eDNA from riverine systems. Journal of Applied Ecology, 574, 707–716. https://doi.org/10.1111/1365-2664.13592

Santoro, E. P., Borges, R. M., Espinoza, J. L., Freire, M., Messias, C. S. M. A., Villela, H. D. M., Pereira, L. M., Vilela, C. L. S., Rosado, J. G., Cardoso, P. M., Rosado, P. M., Assis, J. M., Duarte, G. A. S., Perna, G., Rosado, A. S., Macrae, A., Dupont, C. L., Nelson, K. E., Sweet, M. J., … Peixoto, R. S. (2021). Coral microbiome manipulation elicits metabolic and genetic restructuring to mitigate heat stress and evade mortality. Science Advances, 733, eabg3088. https://doi.org/10.1126/sciadv.abg3088

Saragusty, J., Diecke, S., Drukker, M., Durrant, B., Friedrich Ben‐Nun, I., Galli, C., Göritz, F., Hayashi, K., Hermes, R., Holtze, S., Johnson, S., Lazzari, G., Loi, P., Loring, J. F., Okita, K., Renfree, M. B., Seet, S., Voracek, T., Stejskal, J., … Hildebrandt, T. B. (2016). Rewinding the process of mammalian extinction. Zoo Biology, 354, 280–292. https://doi.org/10.1002/zoo.21284

Scholz, A. H., Freitag, J., Lyal, C. H. C., Sara, R., Cepeda, M. L., Cancio, I., Sett, S., Hufton, A. L., Abebaw, Y., Bansal, K., Benbouza, H., Boga, H. I., Brisse, S., Bruford, M. W., Clissold, H., Cochrane, G., Coddington, J. A., Deletoille, A. C., García-Cardona, F., … Overmann, J. (2022). Multilateral benefit-sharing from digital sequence information will support both science and biodiversity conservation. Nature Communications, 13, 1086. https://doi.org/10.1038/s41467-022-28594-0

Scholz, A. H., Nunez-Vega, G., Weissgold, L., & Wussmann, K. (2023). The future of access and benefit-sharing: what next after the adoption of the global biodiversity framework and decision on digital sequence information? Diversity, 161, 27. https://doi.org/10.3390/d16010027

Shafer, A. B. A., Wolf, J. B. W., Alves, P. C., Bergström, L., Bruford, M. W., Brännström, I., Colling, G., Dalén, L., De Meester, L., Ekblom, R., Fawcett, K. D., Fior, S., Hajibabaei, M., Hill, J. A., Hoezel, A. R., Höglund, J., Jensen, E. L., Krause, J., Kristensen, T. N., … Zieliński, P. (2015). Genomics and the challenging translation into conservation practice. Trends in Ecology & Evolution, 302, 78–87. https://doi.org/10.1016/j.tree.2014.11.009

Sherman, B., & Henry, R. J. (2021). Access to biodiversity for food production: reconciling open access digital sequence information with access and benefit sharing. Molecular Plant, 145, 701–704. https://doi.org/10.1016/j.molp.2021.03.005

Stoeckle, M. Y., Adolf, J., Ausubel, J. H., Charlop-Powers, Z., Dunton, K. J., & Hinks, G. (2022). Current laboratory protocols for detecting fish species with environmental DNA optimize sensitivity and reproducibility, especially for more abundant populations. ICES Journal of Marine Science, 792, 403–412. https://doi.org/10.1093/icesjms/fsab273

Thomsen, P. F., Kielgast, J., Iversen, L. L., Møller, P. R., Rasmussen, M., & Willerslev, E. (2012). Detection of a diverse marine fish fauna using environmental DNA from seawater samples. PLoS ONE, 78, e41732. https://doi.org/10.1371/journal.pone.0041732

Thomsen, P. F., Kielgast, J., Iversen, L. L., Wiuf, C., Rasmussen, M., Gilbert, M. T. P., Orlando, L., & Willerslev, E. (2012). Monitoring endangered freshwater biodiversity using environmental DNA. Molecular Ecology, 21(11), 2565–2573. https://doi.org/10.1111/j.1365-294X.2011.05418.x

Thomsen, P. F., & Sigsgaard, E. E. (2019). Environmental DNA metabarcoding of wild flowers reveals diverse communities of terrestrial arthropods. Ecology and Evolution, 94, 1665–1679. https://doi.org/10.1002/ece3.4809

van der Heijden, M. G. A., Klironomos, J. N., Ursic, M., Moutoglis, P., Streitwolf-Engel, R., Boller, T., Wiemken, A., & Sanders, I. R. (1998). Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature, 396(6706), 69–72. https://doi.org/10.1038/23932

van der Heyde, M., Bunce, M., Wardell‐Johnson, G., Fernandes, K., White, N. E., & Nevill, P. (2020). Testing multiple substrates for terrestrial biodiversity monitoring using environmental DNA metabarcoding. Molecular Ecology Resources, 203, 732–745. https://doi.org/10.1111/1755-0998.13148

van Oppen, M. J. H., Oliver, J. K., Putnam, H. M., & Gates, R. D. (2015). Building coral reef resilience through assisted evolution. Proceedings of the National Academy of Sciences, 112(8), 2307–2313. https://doi.org/10.1073/pnas.1422301112

Wildt, D. E. (2000). Genome resource banking for wildlife research, management and conservation. ILAR Journal, 41(4), 228–234. https://doi.org/10.1093/ilar.41.4.228

Wubs, E. R. J., van der Putten, W. H., Bosch, M., & Bezemer, T. M. (2016). Soil inoculation steers restoration of terrestrial ecosystems. Nature Plants, 2(8), 16107. https://doi.org/10.1038/nplants.2016.107

Yin, K., Chung, M. Y., Lan, B., Du, F. K., & Chung, M. G. (2024). Plant conservation in the age of genome editing: Opportunities and challenges. Genome Biology, 25(1), 279. https://doi.org/10.1186/s13059-024-03399-0

Downloads

Published

2026-09-15

How to Cite

Olukayode, O. J., Okpro, O., West, S. T., & Aver, Z. S. (2026). The Role of Biotechnology in Biodiversity Conservation and Ecological Restoration: A Review. American Journal of Life Science and Innovation, 5(2), 27-35. https://doi.org/10.54536/ajlsi.v5i2.8384