Antioxidant Activities of Selected Milk Teas and Fruit Teas Using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay

Authors

  • Perez, Jeanine Therese V. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Perez, Lyx Jady Fy N. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Padroncillo, Femma Joy A. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Parreño, Kirk Andrew V. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Policarpio, Marti Angela J. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Porras, Alliah Rose B. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Salamat, Adriel Amadei S. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Sararaña, Rachel Joy S. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Secapuri, Lyle Josh C. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Tacluyan, Ann Marie Heather T. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Tosoc, Daiel Dwight G. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Vito, Joel B. Jr. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Perez, Jose Jr. G. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Palmos, R.J. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Salanio, R. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines
  • Leong-On, Ma. Socorro G. Medical Laboratory Science, College of Pharmacy and Medical Technology, University of San Agustin, Iloilo City, Philippines

DOI:

https://doi.org/10.54536/ajlsi.v4i2.5050

Keywords:

2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay, Antioxidant Activity, Fruit Teas, Milk Teas

Abstract

Milk teas and fruit teas are popular as a refreshment, but their percent antioxidant properties need to be determined. In this study, the antioxidant activities of selected commercialized milk teas (Matcha, Okinawa, and Winter Melon) and fruit teas (Green Apple, Kiwi, and Lychee) were determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay in terms of % scavenging activity. In that assay, the plates with milk teas and fruit teas were incubated in the dark and covered with aluminum foil for 30 minutes at room temperature before being measured for absorbance at 570 nm with a microplate reader. They were kept out of the light until they were evaluated for analysis. Within 30 minutes of incubation, the discoloration from purple to yellow was observed. Three (3) trials with three (3) replicates were carried out with negative control (water with sugar and creamer for milk tea and water with sugar for fruit tea) and positive control (Vitamin C). Using one-way ANOVA, the Matcha (mean = 62.9, sd = 2.6) Okinawa (mean = 46.4, sd = 5.9), and Winter Melon (mean = 61.6, sd = 2.8) milkteas have anti-oxidant activities when compared to the negative control (mean = 0.0, sd = 0.0) (p < .05) Both Matcha and Winter Melon have the best antioxidant activities, but not comparable to the positive control, Vitamin C (mean = 98.7; sd = 0.62) (p < .05). While the Green apple fruit tea (mean = 95.5; SD = 2.4) Kiwi fruit tea (mean = 97.3, SD = 1.5), and Lychee fruit tea (mean = 94.4; sd = 1.3) (p > .05) have comparable antioxidant activities when compared to the negative control (mean sd = 0.0). Kiwi fruit tea has the best antioxidant activity and comparable to Vitamin C (mean = 98.7; sd = 0.62). This study scientifically established that both Matcha and Winter Melon commercial milk teas have antioxidant activities of 61.6 to 62.9%. While the commercial Kiwi, Green apple, and Lychee fruit tea ranged from 94.4 to 97.3%. These antioxidant activities can be useful in preventing diseases brought by free radicals, which can be verified by conducting more studies.

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References

Aksoy, I. S., & Ötleş, S. (2022). Effects of green Apple (Golden Delicious) and its three major flavonols consumption on obesity, lipids, and oxidative stress in obese rats. Molecules, 27(4), 1243.

Bag, S., Mondal, A., Majumder, A., & Banik, A. (2021). Tea and its phytochemicals: hidden health benefits & modulation of signaling cascade by phytochemicals. Food Chemistry, 371, 131098. https://doi.org/10.1016/j.foodchem.2021.131098

Bastasa, K. D. S., Maravilla, V. S., Jr., Espellita, S.R., Jr., Calzada, R. D., Alqueza, J. M. (2022, July 4). The popularity of milk tea amidst COVID-19 pandemic: perspectives of selected entrepreneurs in Minglanilla, Cebu, Philippines. https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4152997

Kinjo, Y., Takahashi, M., Hirose, N., Mizu, M., Hou, D., & Wada, K. (2019b). Anti-stress and antioxidant effects of non centrifuged cane sugar, Kokuto, in Restraint-Stressed mice. Journal of Oleo Science, 68(2), 183–191. https://doi.org/10.5650/jos.ess18198

Kochman, J., Jakubczyk, K., Antoniewicz, J., Mruk, H., & Janda, K. (2020). Health benefits and chemical composition of matcha green tea: a review. Molecules, 26(1), 85. https://doi.org/10.3390/molecules26010085

Koláčková, T., Kolofiková, K., Sytařová, I., Snopek, L., Sumczynski, D., & Orsavová, J. (2019). Matcha Tea: Analysis of nutritional composition, phenolics and antioxidant activity. Plant Foods for Human Nutrition, 75(1), 48–53. https://doi.org/10.1007/s11130-019- 00777-z

Kumari, S., Manohar, S., Kumari, P., Krishnan, V., Maheshwari, C., Narwal, S., Gupta, O. P., Gowda, V. T., Bansal, N., & Dahuja, A. (2023). The role of major phenolics in Apple to total antioxidant capacity. In IntechOpen eBooks. https://doi.org/10.5772/intechopen.109064

Munteanu, I. G., & Apetrei, C. (2021). Analytical methods used in determining antioxidant Activity: a review. International Journal of Molecular Sciences, 22(7), 3380. https://doi.org/10.3390/ijms22073380

Musial, C., Kuban-Jankowska, A., & Gorska-Ponikowska, M. (2020). Beneficial properties of green tea catechins. International Journal of Molecular Sciences, 21(5), 1744-1745. https://doi.org/10.3390/ijms21051744

National Nutrition Council. (2020, September 16). Milk Tea: Sip it or Skip it. https://nnc.gov.ph/regional-offices/mindanao/region-x-northern-mindanao/4088-milk-tea- sip-it-or-skip-it

Ong, A. K. S., Prasetyo, Y. T., Libiran, M. a. D. C., Lontoc, Y. M. A., Lunaria, J. a. V., Manalo, A. M., Miraja, B. A., Young, M. N., Chuenyindee, T., Persada, S. F., & Redi, A. a. N. P. (2021). Consumer Preference Analysis on Attributes of Milk Tea: A Conjoint Analysis approach. Foods, 10(6), 1382. https://doi.org/10.3390/foods10061382

Purohit, P., Palamthodi, S., & Lele, S. S. (2019). Effect of karwanda (Carissa congesta Wight) and sugar addition on physicochemical characteristics of ash gourd (Benincasa hispida) and bottle gourd (Langenaria siceraria) based beverages. Journal of Food Science and Technology, 56(2), 1037–1045. https://doi.org/10.1007/s13197-019-03570-7

Richardson, D., Ansell, J., & Drummond, L. (2018). The nutritional and health attributes of kiwifruit: a review. European Journal of Nutrition, 57(8), 2659–2676. https://doi.org/10.1007/s00394-018-1627-z

Şahin, S. (2013). Evaluation of antioxidant properties and phenolic composition of fruit tea infusions. Antioxidants, 2(4), 206–215. https://doi.org/10.3390/antiox2040206

Saiful, A. (2022, June 20). Okinawa Milk Tea Recipe From Scratch Or Powder Form. Honest Food Talks. https://www.honestfoodtalks.com/okinawa-milk-tea-recipe/

Sharma, P., Rani, R., Ganie, S. Y., Sheikh, T. A., Javaid, D., Qadri, S. S., Pramodh, S., Alsulimani, A., Alkhanani, M., Harakeh, S., Hussain, A., Haque, S., & Reshi, M. S. (2022). Oxidative Stress in Human Pathology and aging: Molecular mechanisms and perspectives. Cells, 11(3), 552. https://doi.org/10.3390/cells1103055

Siddique, A. B., Idrees, N., Kashif, M., Ahmad, R., Ali, A., Siddiqua, A. A., & Javied, M. (2021). Antibacterial and Antioxidant Activity of Kiwi Fruit. Biological & Clinical Sciences Research Journal, 2021(1). https://doi.org/10.54112/bcsrj.v2021i1.76

Sokary, S., Al-Asmakh, M., Zakaria, Z. Z., & Bawadi, H. (2023). The therapeutic potential of matcha tea: A critical review on human and animal studies. Current Research in Food Science, 6, 100396. https://doi.org/10.1016/j.crfs.2022.11.015

Stobiecka, M., Król, J., & Brodziak, A. (2022). Antioxidant activity of milk and dairy products. Animals, 12(3), 245. https://doi.org/10.3390/ani12030245

Szerlauth, A., Muráth, S., Viski, S., & Szilágyi, I. (2019). Radical scavenging activity of plant extracts from improved processing. Heliyon, 5(11), e02763. https://doi.org/10.1016/j.heliyon.2019.e02763

Xiao, F., Xu, T., Lu, B., & Liu, R. (2020). Guidelines for antioxidant assays for food components. Food Frontiers, 1(1), 60–69. https://doi.org/10.1002/fft2.10

Yoo, S., Kim, K., Nam, H., & Lee, D. (2018). Discovering health benefits of phytochemicals with integrated analysis of the molecular network, chemical properties, and ethnopharmacological evidence. Nutrients, 10(8). https://doi.org/10.3390/nu10081042

Zhang, J., Tian, J., Gao, N., Gong, E. S., Xin, G., Liu, C., Si, X., Sun, X., & Li, B. (2021). Assessment of the phytochemical profile and antioxidant activities of eight kiwi berry (Actinidia arguta (Siebold & Zuccarini) Miquel) varieties in China. Food Science & Nutrition, 9(10), 5616–5625. https://doi.org/10.1002/fsn3.2525

Zhang, Y., Jin, D., An, X., Duan, L., Duan, Y., & Lian, F. (2021). Lychee Seed as a Potential Hypoglycemic Agent, and Exploration of its Underlying Mechanisms. Frontiers in Pharmacology, 12. https://doi.org/10.3389/fphar.2021.737803

Zieniewska, I., Zalewska, A., Żendzian-Piotrowska, M., Ładny, J. R., & Maciejczyk, M. (2020). Antioxidant and Antiglycation Properties of Seventeen Fruit Teas Obtained from One Manufacturer. Applied Sciences, 10(15), 5195. https://doi.org/10.3390/app10155195

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Published

2025-10-11

How to Cite

Perez, J. T. V., Perez, L. J. F. N., Padroncillo, F. J. A., Parreño, K. A. V., Policarpio, M. A. J., Porras, A. R. B., Salamat, A. A. S., Sararaña, R. J. S., Secapuri, L. J. C., Tacluyan, A. M. H. T., Tosoc, D. D. G., Vito, J. B. J., Perez, J. J. G., Palmos, R., Salanio, R., & Leong-on, M. S. G. (2025). Antioxidant Activities of Selected Milk Teas and Fruit Teas Using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay. American Journal of Life Science and Innovation, 4(2), 6–10. https://doi.org/10.54536/ajlsi.v4i2.5050

Issue

Vol. 4 No. 2 (2025): American Journal of Life Science and Innovation

Section

Research Articles

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Copyright (c) 2025 Perez, Jeanine Therese V., Perez, Lyx Jady Fy N., Padroncillo, Femma Joy A., Parreño, Kirk Andrew V., Policarpio, Marti Angela J., Porras, Alliah Rose B., Salamat, Adriel Amadei S., Sararaña, Rachel Joy S., Secapuri, Lyle Josh C., Tacluyan, Ann Marie Heather T., Tosoc, Daiel Dwight G., Vito, Joel B. Jr., Perez, Jose Jr. G., Palmos, R.J., Salanio, R., Leong-On, Ma. Socorro G.

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This work is licensed under a Creative Commons Attribution 4.0 International License.