Response Surface Methodology (RSM) for Optimizing Ultrasound Assisted Extraction of Bioactive Phenolic from Artemisia Annua L (Asteraceae) and Kinetic Approach
DOI:
https://doi.org/10.54536/ajise.v5i2.6279Keywords:
Artemisia annua, Central Composite Design, Kinetics model, Optimization, Response Surface MethodologyAbstract
The objective of this work is to apply a sustainable extraction technique like ultrasound-assisted extraction (UAE) to optimize experimental conditions, with the aim of maximizing the recovery of total phenolic compounds from Artemisia annua leaves. To explain the phenology of the mass transfer in this extraction process, Hervas and Peleg kinetic models were investigated. Single, interaction, and quadratic effects of three independent variables on extraction yields were evaluated using a central composite design through a response surface methodology. The extraction process was carried out successfully under optimal conditions of 15.97 min for extraction times; 68.99 °C for the temperature and a liquid-to-solid ratio of 40:1 mLg-1. Among the several extraction solvents and techniques investigated during extraction process, UAE and ethanol produced the best results. The UAE provides a promising method to obtain the maximum extraction yield of 20.02 % and the best recovery of total phenolics content (TPC) of (76.28 ± 0.07) mg EAG/g of extract). Regarding the optimization results, liquid-to-solid ratio was the most significant factor influencing the extraction yield. Moreover, the extraction time and liquid-to-solid ratio were the two important extraction parameters influencing the extraction kinetics. The suitable kinetics models was the Peleg’s mathematical with the maximum extraction quantities of secondary metabolites Co (0.013 mg EAG/g DW) and the extraction rate coefficients k (0.012 s-1). The goodness of the fit was controlled by the magnitude of the coefficient of determination (R2), and the square root of the mean square error (RMSE).
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